Lighting the way: Sensors show drug uptake

August 2, 2017, American Chemical Society

When designing and characterizing new drugs, a key aspect is making sure the drug actually goes where it is intended to. But current tests for drug uptake monitor the process under unrealistic conditions and do not provide information on the amounts of drugs that cross into a cell. Now, one group reports in ACS Sensors that fluorescent detector proteins can overcome these challenges.

Kai Johnsson and colleagues developed a biosensor equipped with a drug target and two . When the drug was absent from a cell, the proteins fluoresced in a certain color, and when the drug was present, the color changed.

The sensor could be located in the cell, allowing the researchers to monitor drug uptake. Biosensors for two classes of were generated, including one associated with carbon dioxide transport and pH homeostasis in the human body. The researchers say the detection method can be adapted to other enzymes of interest to the pharmaceutical industry.

Explore further: Antibody biosensor offers unlimited point-of-care drug monitoring

More information: "Evaluating Cellular Drug Uptake with Fluorescent Sensor Proteins" ACS Sensors, pubs.acs.org/doi/abs/10.1021/acssensors.7b00331

Related Stories

Biosensors light up cellular signaling processes

July 17, 2017

Researchers at the University of California, Riverside have converted a naturally occurring fluorescent protein from corals into a biosensor that can be used to monitor the cellular thioredoxin (Trx) system, which is a promising ...

Drug delivery that hits the dot

May 24, 2016

Drug treatments are made more efficient by delivering them to specific sites in the body where they are needed. For example, specific targeting of anticancer drugs to tumour sites could reduce required doses, provide more ...

New mechanism to fight multi-resistant bacteria revealed

April 19, 2017

In recent years, scientists, clinicians and pharmaceutical companies have struggled to find new antibiotics or alternative strategies against multi-drug resistant bacteria that represent a serious public health problem. In ...

Recommended for you

The changing shape of DNA

May 24, 2018

The shape of DNA can be changed with a range of triggers including copper and oxygen—according to new research from the University of East Anglia.

Universal pH regulated assembly of DNA nanostructures

May 23, 2018

DNA, the carrier of genetic information, has become established as a highly useful building material in nanotechnology. One requirement in many applications is the controlled, switchable assembly of nanostructures. In the ...

0 comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.