Lighting the way: Sensors show drug uptake

August 2, 2017, American Chemical Society

When designing and characterizing new drugs, a key aspect is making sure the drug actually goes where it is intended to. But current tests for drug uptake monitor the process under unrealistic conditions and do not provide information on the amounts of drugs that cross into a cell. Now, one group reports in ACS Sensors that fluorescent detector proteins can overcome these challenges.

Kai Johnsson and colleagues developed a biosensor equipped with a drug target and two . When the drug was absent from a cell, the proteins fluoresced in a certain color, and when the drug was present, the color changed.

The sensor could be located in the cell, allowing the researchers to monitor drug uptake. Biosensors for two classes of were generated, including one associated with carbon dioxide transport and pH homeostasis in the human body. The researchers say the detection method can be adapted to other enzymes of interest to the pharmaceutical industry.

Explore further: Antibody biosensor offers unlimited point-of-care drug monitoring

More information: "Evaluating Cellular Drug Uptake with Fluorescent Sensor Proteins" ACS Sensors, pubs.acs.org/doi/abs/10.1021/acssensors.7b00331

Related Stories

Biosensors light up cellular signaling processes

July 17, 2017

Researchers at the University of California, Riverside have converted a naturally occurring fluorescent protein from corals into a biosensor that can be used to monitor the cellular thioredoxin (Trx) system, which is a promising ...

Drug delivery that hits the dot

May 24, 2016

Drug treatments are made more efficient by delivering them to specific sites in the body where they are needed. For example, specific targeting of anticancer drugs to tumour sites could reduce required doses, provide more ...

New mechanism to fight multi-resistant bacteria revealed

April 19, 2017

In recent years, scientists, clinicians and pharmaceutical companies have struggled to find new antibiotics or alternative strategies against multi-drug resistant bacteria that represent a serious public health problem. In ...

Recommended for you

In colliding galaxies, a pipsqueak shines bright

February 20, 2019

In the nearby Whirlpool galaxy and its companion galaxy, M51b, two supermassive black holes heat up and devour surrounding material. These two monsters should be the most luminous X-ray sources in sight, but a new study using ...

When does one of the central ideas in economics work?

February 20, 2019

The concept of equilibrium is one of the most central ideas in economics. It is one of the core assumptions in the vast majority of economic models, including models used by policymakers on issues ranging from monetary policy ...

Research reveals why the zebra got its stripes

February 20, 2019

Why do zebras have stripes? A study published in PLOS ONE today takes us another step closer to answering this puzzling question and to understanding how stripes actually work.

Correlated nucleons may solve 35-year-old mystery

February 20, 2019

A careful re-analysis of data taken at the Department of Energy's Thomas Jefferson National Accelerator Facility has revealed a possible link between correlated protons and neutrons in the nucleus and a 35-year-old mystery. ...

0 comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.