Artificial fluorescent membrane lipid shows active role in living cells

Artificial fluorescent membrane lipid shows active role in living cells
(top) Newly synthesized sphingomyelin fluorescent analogue molecule. (Bottom) Differences from conventional fluorescent analog lipids. By using hydrophilic fluorescent molecule and linker, the same behavior as natural lipid can be reproduced. Credit: Osaka University

Biological membranes, such as those surrounding animal cells, are made up of lipids and proteins. Because these molecules do not usually mix well, they are distributed within different regions of the membrane. This segregation is achieved in a number of ways, including the formation of domains based on particular lipids such as cholesterol or sphingomyelin (SM). These two lipids are required for the generation of cholesterol-dependent raft domains, which are necessary for signaling within the plasma membrane. However, it was not clear how SMs interacted with other molecules of raft domains, mainly because of the lack of a suitable synthetic probe of SM. Now, research led by Osaka University in collaboration with JST ERATO Lipid Active Structure Project has developed new fluorescent synthetic molecules (analogs) that structurally mimic SMs and can be studied in live cells. The study was reported in Journal of Cell Biology.

Existing fluorescent SM analogs behave differently from their fully functional natural counterparts. For example, they usually separate into a different type of fluid phase from that seen in living membranes. Moreover, those synthetic analogs that do split into the correct fluid phase produce a weak fluorescent signal, quickly lose their pigment, or sometimes need to be excited by UV light.

Researchers at Osaka University overcame these limitations with fluorescent SM analogs by joining several fluorescent chemical compounds (fluorophores) that were highly hydrophilic to the hydrophobic lipid part (mainly acyl chains) of the synthetic molecule. "We took care to ensure that the positive charge of the headgroup was maintained by not modifying its lipid part," co-first author Masanao Kinoshita says. "This was achieved by keeping the fluorescent compounds away from the headgroup using a long linker component."

After confirming that the synthetic behaved similarly to natural SM by using simple model membranes, the team next used highly sensitive single-molecule imaging to monitor the role of SMs in living cell membranes.

Artificial fluorescent membrane lipid shows active role in living cells
The green fluorescent sphingomyelin molecule is localized in the raft-like region of the artificial membrane. The red area is a non-raft membrane area. Credit: Osaka University

"We observed interactions of the SM analogs with each other and with CD59, which is a type of receptor that is commonly used to link proteins to the ," corresponding author Nobuaki Matsumori says. "These interactions were shown to sometimes require the presence of cholesterol as well as an alcohol component of SMs."

Further analysis revealed the dynamic behavior of SMs as they rapidly associated and dissociated from raft domains involving different formations of CD59 and with the plasma . These findings may help in modifying future molecular interactions such as increasing their rate or complexity.

Artificial fluorescent membrane lipid shows active role in living cells
A schematic diagram of transient binding of sphingomyelin to various forms of CD59, which was clarified in this study. Credit: (c) 2017 Kinoshita M. et al. Journal of Cell Biology. VOL:216 NO:4 1183-1204. doi: 10.1083/jcb.201607086

Explore further

Membrane lipids hop in and out of rafts in the blink of an eye

More information: Masanao Kinoshita et al, Raft-based sphingomyelin interactions revealed by new fluorescent sphingomyelin analogs, The Journal of Cell Biology (2017). DOI: 10.1083/jcb.201607086
Journal information: Journal of Cell Biology

Provided by Osaka University
Citation: Artificial fluorescent membrane lipid shows active role in living cells (2017, June 5) retrieved 23 October 2019 from
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.

Feedback to editors

User comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more