Endocrine disrupting chemicals (EDCs) have been implicated in the development of obesity, diabetes and cancer and are found in a wide array of products including pesticides, plastics and pharmaceuticals. EDCs are potentially harmful, even at low concentrations, equal in some cases to mere milligrams dissolved in in a swimming pool full of water. Now researchers report in ACS Central Science that they can quickly detect environmentally relevant concentrations of EDCs using engineered E. coli bacteria.
Detecting EDCs can be tough because the classification is based on their activity—disrupting hormone function—instead of their structures. Thus the term encompasses a broad spectrum of chemicals and often, health risks arise from aggregate exposure to several different species. Because many EDCs act on the same hormone receptors on a cell's surface, researchers have been developing tests that detect the compounds based on their ability to interfere with hormones. But these methods currently take days to produce a result or involve many complicated and expensive steps. Here, Matthew Francis and colleagues overcame these challenges by using E. coli in their device.
Non-toxic, dead E. coli cells display an estrogen receptor on the surface of the researchers' portable sensor. A protein on the sensor surface recognizes the EDC-E. coli complex, producing an electronic read-out in minutes. The inexpensive device can determine the concentration of many known EDCs individually and overall concentrations as mixtures. They tested the detection in water and in complex solutions like baby formula. It also can detect EDCs released into liquid from a plastic baby bottle following microwave heating. The team notes that their test is suitable for use in the field and can be modified to test for other types of chemicals that act on human receptors.
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More information: Ariel L. Furst et al. Quantifying Hormone Disruptors with an Engineered Bacterial Biosensor, ACS Central Science (2017). DOI: 10.1021/acscentsci.6b00322
Endocrine disrupting compounds are found in increasing amounts in our environment, originating from pesticides, plasticizers, and pharmaceuticals, among other sources. Although the full impact of these compounds is still under study, they have already been implicated in diseases such as obesity, diabetes, and cancer. The list of chemicals that disrupt normal hormone function is growing at an alarming rate, making it crucially important to find sources of contamination and identify new compounds that display this ability. However, there is currently no broad-spectrum, rapid test for these compounds, as they are difficult to monitor because of their high potency and chemical dissimilarity. To address this, we have developed a new detection strategy for endocrine disrupting compounds that is both fast and portable, and it requires no specialized skills to perform. This system is based on a native estrogen receptor construct expressed on the surface of Escherichia coli, which enables both the detection of many detrimental compounds and signal amplification from impedance measurements due to the binding of bacteria to a modified electrode. With this approach, sub-ppb levels of estradiol and ppm levels of bisphenol A are detected in complex solutions. Rather than responding to individual components, this system reports the total estrogenic activity of a sample using the most relevant biological receptor. As an applied example, estrogenic chemicals released from a plastic baby bottle following microwave heating were detectable with this technique. This approach should be broadly applicable to the detection of chemically diverse classes of compounds that bind to a single receptor.