Dormancy relief, storage protocols for uhaloa seeds
Uhaloa (Waltheria indica) is a pantropical shrub species found in the Americas, Mexico, and Brazil. In Hawaii, uhaloa is widely classified as a native plant. A team of scientists at the University of Hawaii at Manoa published a research study that contains recommendations for long-term storage and utilization of uhaloa from seed stock. The protocols can inform sustainability methods for uhaloa, a plant that has been identified for expanded use as a roadside groundcover in lowland dry ecosystems.
Scott Lukas, Joseph DeFrank, and Orville Baldos from the Department of Tropical Plant and Soil Sciences at the University of Hawaii at Manoa designed a study (HortScience, September 2106) to determine physical dormancy breaking mechanisms of uhaloa seeds in which they evaluated seed germination in response to manual and mechanical scarification and exposure to dry heat and hot water emersion. The study also assessed the impact of storage humidity and duration at 5 °C on viability and germination response of nonscarified and scarified seeds.
The researchers tested uhaloa seeds after hand scarification, dry heat temperature exposure, hot water exposure, and mechanical abrasion in an electric drum scarifier. "The elucidation of physical dormancy was determined through hand scarification, resulting in 96% germination compared with 8% of nonscarified seeds, but is not practical on a large-scale basis," said corresponding author Scott Lukas. The greatest "practical dormancy relief" was achieved using a mechanical electric drum scarifier lined with 80-grit sandpaper for a duration of 15 or 30 seconds, which produced 95% and 99% germination, respectively. Seeds immersed in boiling water for 3 and 5 seconds resulted in 58.6% and 57.7% germination, respectively. Additional analyses showed that dormancy relief through dry heat exposure was inferior to other relief methods, producing 39% germination at 75 °C for 60 minutes.
Nonscarified seeds exhibited minimal loss of viability during 10 months of storage at 5 °C at 12% and 50% relative humidity (RH), but a significant decline in viability of scarified seeds was detected.
The authors recommended protocols for the utilization of uhaloa from seed stock. "If seeds are not needed immediately after harvest, store at 5 °C at either 12% or 50% RH. When seeds are desired for use, scarify using an electric drum scarifier with 80-grit sandpaper for 30 seconds to relieve physical dormancy. Once the seeds are scarified, viability will decline significantly after 2 months of storage at low temperatures," the authors said.