Super-resolution microscope builds 3-D images by mapping negative space

Super-resolution microscope builds 3-D images by mapping negative space
Scientists at The University of Texas at Austin have developed a new microscopy technique for looking at nanoscale structures in biological samples that is analogous to using a glowing rubber ball to image a chair in a dark room. Credit: Illustration by Jenna Luecke

Scientists at The University of Texas at Austin have demonstrated a method for making three-dimensional images of structures in biological material under natural conditions at a much higher resolution than other existing methods. The method may help shed light on how cells communicate with one another and provide important insights for engineers working to develop artificial organs such as skin or heart tissue.

The research is described today in the journal Nature Communications.

The scientists, led by physicist Ernst-Ludwig Florin, used their method, called thermal noise imaging, to capture nanometer-scale images of networks of collagen fibrils, which form part of the connective tissue found in the skin of animals. A nanometer is a billionth of a meter or about one-hundred-thousandth of the width of a human hair. Examining collagen fibrils at this scale allowed the scientists to measure for the first time key properties that affect skin's elasticity, something that could lead to improved designs for artificial skin or tissues.

Taking crisp 3-D images of nanoscale structures in is extremely difficult, in part because they tend to be soft and bathed in liquid. This means that tiny fluctuations in heat cause structures to move back and forth, an effect known as Brownian motion.

To overcome the blurriness that this creates, other super-resolution imaging techniques often "fix" biological samples by adding chemicals that stiffen various structures, in which case, materials lose their natural mechanical properties. Scientists can sometimes overcome blurriness without fixing the samples if, for example, they focus on rigid structures stuck to a glass surface, but that severely limits the kinds of structures and configurations they can study.

Florin and his team took a different approach. To make an image, they add nanospheres - nanometer-sized beads that reflect laser light - to their biological samples under , shine a laser on the sample and compile superfast snapshots of the nanospheres viewed through a light microscope.

The scientists explain that the method, thermal noise imaging, works something like this analogy: Imagine you needed to take a three-dimensional image of a room in total darkness. If you were to throw a glowing rubber ball into the room and use a camera to collect a series of high-speed images of the ball as it bounces around, you would see that as the ball moves around the room, it isn't able to move through solid objects such as tables and chairs. Combining millions of images taken so fast that they don't blur, you would be able to build a picture of where there are objects (wherever the ball couldn't go) and where there aren't objects (where it could go).

In thermal noise imaging, the equivalent of the rubber ball is a nanosphere that moves around in a sample by natural Brownian motion - the same unruly force that has bedeviled other microscopy methods.

"This chaotic wiggling is a nuisance for most microscopy techniques because it makes everything blurry," says Florin. "We've turned it to our advantage. We don't need to build a complicated mechanism to move our probe around. We sit back and let nature do it for us."

The original concept for the imaging technique was published and patented in 2001, but technical challenges prevented it from being developed into a fully functioning process until now.

The tool allowed the researchers to measure for the first time the of in a network. Collagen is a biopolymer that forms scaffolds for cells in the skin and contributes to the skin's elasticity. Scientists are still not sure how a collagen network's architecture results in its elasticity, an important question that must be answered for the rational design of artificial skin.

"If you want to build , you have to understand how the natural components work," says Florin. "You could then better design a collagen network that acts as a scaffolding that encourages cells to grow in the right way."


Explore further

Newly discovered features of collagen may help shed light on disease processes

More information: Nature Communications, DOI: 10.1038/NCOMMS12729
Journal information: Nature Communications

Citation: Super-resolution microscope builds 3-D images by mapping negative space (2016, September 6) retrieved 26 August 2019 from https://phys.org/news/2016-09-super-resolution-microscope-d-images-negative.html
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.
1422 shares

Feedback to editors

User comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more