Rapid test identifies disease pathogens

July 1, 2016, Fraunhofer-Gesellschaft
Production of ImmuStick: Application of immune receptors onto the test strip. Credit: Fraunhofer IGB

At present, bacteria, fungi or viruses can generally only be detected with certainty by way of elaborate laboratory tests or animal experiments. The food and pharmaceutical industries would like to have faster tests to check their products. Fraunhofer researchers are therefore developing a stick that works like a pregnancy test and quickly delivers a result. In the future, it is also to be used for detecting allergens and disease pathogens in the blood.

Researchers at the Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB in Stuttgart are developing a which rapidly and cost-effectively identifies bacteria, fungi or viruses. It can be carried out directly in situ without laboratory equipment and specialist knowledge. "The ImmuStick can even detect pathogens outside the body – on medical devices or in hospital rooms for example. However, the technology would certainly also be of interest for testing human blood for germs or allergies", says Dr. Anke Burger-Kentischer.

As easy as a pregnancy test

The method works as simply as a pregnancy test. The ImmuStick is a test strip onto which a few drops of fluid are applied. If the fluid contains pyrogens, fragments of pathogens, this is shown by a colored strip in a viewing window. First of all, human immune receptors sensitive to certain pyrogens are applied to the surface of the stick. These are laboratory-produced immune receptors which are synthesized on the basis of the biological model. During production, at the docking point of the immune receptors to which the pyrogens normally bind, a type of placeholder is mounted which is marked with a dye. When drops of a fluid containing pyrogens are then applied to the test strip, the pyrogens rush to the docking point on the immune receptor. The placeholders marked with the dye migrate with the fluid through the test strip until they are visible in the viewing window. The color signal thus indicates that pyrogens that have docked on the immune receptors are present.

The ImmuStick project was financed with money from the Discover program. In this way the Fraunhofer-Gesellschaft is supporting projects for the duration of one year in order to demonstrate the feasibility of a technology. The ImmuStick has passed this test. "We were able to show that it works very well for the bacterial pyrogen LPS. Together with industrial partners, we now want to develop it into a product", says project manager Burger-Kentischer. "We are currently testing further that are specific for other pyrogens."

Detecting blood poisoning and allergies

Currently envisaged are applications in the food and pharmaceuticals sector or in medical technology, as a complete absence of germs or pyrogens is required there. In principle, the ImmuStick would also be of interest for blood analysis. Pyrogens in the blood often lead to blood poisoning, sepsis, from which many people still die today, especially weakened intensive care patients. "However, blood is a special challenge as it is complex and contains many constituent parts. But in the medium term we are aiming at blood analysis", says Burger-Kentischer.

As pyrogens also include certain allergy trigger factors, an application here would also be conceivable. In the food and pharmaceutical industries, for example, it is important that products are free of allergens. With the ImmuStick these could be detected quickly, cost-effectively and simply. Costly and laborious laboratory tests would therefore no longer be needed or could be supplemented. At present the IGB researchers are seeking cooperation partners who want to further develop the ImmuStick to make it ready for the market.

Pyrogens become a problem when hygiene is of particular importance – in the food and pharmaceutical industries for example, or on intensive care wards in hospitals. Especially people with weakened immune systems can become severely ill. For this reason, tests are frequently carried out and the surfaces of machines or medical devices are tested for pyrogens using swabs. However, to date these tests have been costly and laborious as pyrogens can only be detected with laboratory equipment. A widely used standard test is the detection of LPS, a structure that is present in the membrane of certain bacteria. At present this test takes up around two hours. Other pyrogens can even only be detected in animal experiment.

Pyrogens versus the immune system

Like a security service, our immune system guards against penetration by foreign bodies. Just fragments of bacteria, fungi or viruses are sufficient to put the immune system on alert. Such pathogen fragments are called pyrogens – "fire generators." The body's immune defense system reacts to them in the form of inflammations and fever. Pyrogens are recognized by way of certain molecular structures, which, for example, are often present in the bacterial membrane. The human immune system has inherent receptors which fit into these molecular structures like a key in a lock.

Explore further: Researchers find more uses for immune system's 'Swiss army knife'

Related Stories

Receptor suppresses the immune response in order to save it

June 29, 2016

When viruses enter the body, they activate receptors on the surface of cells that allow viruses to invade those cells. A Yale-led team has found that one of the receptors, known as AXL, actually plays an essential role in ...

New blood test for the detection of bovine TB

May 31, 2016

A new blood test to detect Mycobacteria in blood has been developed by a team at The University of Nottingham led by Dr Cath Rees, an expert in microbiology in the School of Biosciences and Dr Ben Swift from the School of ...

New model of T cell activation

May 27, 2016

T cell receptors are an important part of the human immune system. They are able to switch their conformation from an inactive to an active state spontaneously without any antigens present. Cholesterol binds and stabilizes ...

Recommended for you

What happened before the Big Bang?

March 26, 2019

A team of scientists has proposed a powerful new test for inflation, the theory that the universe dramatically expanded in size in a fleeting fraction of a second right after the Big Bang. Their goal is to give insight into ...

Probiotic bacteria evolve inside mice's GI tracts

March 26, 2019

Probiotics—which are living bacteria taken to promote digestive health—can evolve once inside the body and have the potential to become less effective and sometimes even harmful, according to a new study from Washington ...

Cellular microRNA detection with miRacles

March 26, 2019

MicroRNAs (miRNAs) are short noncoding regulatory RNAs that can repress gene expression post-transcriptionally and are therefore increasingly used as biomarkers of disease. Detecting miRNAs can be arduous and expensive as ...

0 comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.