X-rays reveal the self-defence mechanisms of bacteria

X-rays reveal the self-defence mechanisms of bacteria
The toxins normally bind very strongly to the antitoxins and are thus not only inactive, but also prevent the production of more toxin from the information encoded in the bacterial DNA. During the dormant state, however, the antitoxins are degraded, and the toxins released (step 1). The free toxins now bind to unoccupied antitoxins on DNA within the area encoding the toxin-antitoxin couple (step 2). Binding increasing amounts of toxin eventually leads to the release of the molecules from the gene (steps 3 and 4) and finally to new toxin production. Credit: Ditlev E. Brodersen

A research group at Aarhus University has gained unique insight into how bacteria control the amount of toxin in their cells. The new findings can eventually lead to the development of novel forms of treatment for bacterial infections.

Many pathogenic bacteria are able to go into a dormant state by producing persister cells that are not susceptible to conventional antibiotics. This causes serious problems in the treatment of life-threatening diseases such as tuberculosis, where the presence of persister cells often leads to a resurgence of infection following .

At the molecular level, the formation of persister cells is due to the presence of toxins that are produced by the bacteria themselves, and which enable them to enter the dormant state. During this hibernation period, the bacteria constantly regulate the amount of toxin at exactly the same level and thus maintain the dormant state.

In an article recently published in the American scientific journal Structure, the researchers at the Department of and Genetics, Aarhus University, present new results that reveal the molecular details of the of toxins.

By isolating and crystallising the toxin molecules and their molecular companions – the antitoxins – and by subsequently exposing the crystals to strong X-rays, the research team (consisting of the two PhD students Andreas Bøggild and Nicholas Sofos and Associate Professor Ditlev E. Brodersen) gained unique insight into how bacteria control the amount of toxin in the cell (illustrated in the info box).

The new findings can eventually lead to the development of entirely new forms of treatment of bacterial infections that work initially by blocking function and production, and subsequently by using traditional antibiotics to fight the .


Explore further

Addressing the challenge of persister cells in bacterial infections

More information: www.sciencedirect.com/science/ … ii/S0969212612002997
Journal information: Structure

Provided by Aarhus University
Citation: X-rays reveal the self-defence mechanisms of bacteria (2012, September 14) retrieved 18 October 2019 from https://phys.org/news/2012-09-x-rays-reveal-self-defence-mechanisms-bacteria.html
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