Resistant wheat goes for the gut to protect against Hessian flies

Feb 08, 2010 by Brian Wallheimer

(PhysOrg.com) -- Resistant wheat plants stave off attacks by Hessian fly larvae by essentially destroying the fly's midgut and its ability to absorb nutrients, according to a study by Purdue University and the U.S. Department of Agriculture.

Hessian fly larvae midguts - similar to human intestines - were observed in three different feeding situations. Larvae on susceptible plants had normal midgut function. Those that were given nothing to eat showed no damage to the midgut, though they starved. But those on plants resistant to Hessian flies showed serious midgut disruption.

Richard Shukle and Christie Williams, research scientists with the USDA Agricultural Research Service Crop Production and Pest Control Research Unit and Purdue adjunct associate professors of entomology, and Subhashree Subramanyam, a research associate in agronomy, had their findings published in the early online edition of the Journal of Insect Physiology.

Shukle said that within three hours, larvae feeding on resistant wheat had abnormal microvilli, fingerlike appendages that extend inward from the walls of the midgut to increase surface area for nutrient absorption. By six hours, the microvilli were nearly destroyed and the midgut was void of food.

"Some chemical or compound they're encountering from the resistant plant is causing the microvilli to become disrupted, and it's happening very quickly," Shukle said. "The midgut is certainly one of the major targets of the defense compounds elicited from a resistant plant. There could be other targets, but the midgut is a major target."

The research also showed that Hessian fly midguts are lacking a peritrophic membrane, a common insect feature that protects the microvilli from toxic substances.

Hessian flies cause considerable damage to wheat crops, with grain yields reduced as much as 20 bushels per acre with moderate infestation levels. There are about a half dozen undeployed genes identified in wheat that offer high resistance to Hessian flies and could be deployed to defend the plants. But understanding how the resistance genes stave off Hessian flies is important because the fly has overcome or is overcoming several genes initially deployed to protect wheat lines.

Williams and Subramanyam believe lectins could be one of the toxic substances causing the midgut disruption. Lectins are proteins that bind carbohydrates.

Williams said resistant wheat plants contain higher levels of proteins made by the Hessian fly responsive genes, called Hfr-1 and Hfr-3, than susceptible plants. Studies have shown that the protein made by Hfr-1 is a lectin, and the protein made by Hfr-3 is lectin-like.

The fly larvae activate the genes responsible for resistance, and Williams and Subramanyam believe those may be triggering lectin function.

"Lectins are one of the toxins in resistant , and they are probably targeting the microvilli," Subramanyam said.

Shukle said he will next try to determine what is attaching to the microvilli causing the disruption and will test the Hfr-1 protein to see if it is toxic to the flies.

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More information: Ultrastructural Changes in the Midguts of Hessian Fly Larvae Feeding on Resistant Wheat, Richard H. Shukle, Subhashree Subramanyam, Kristin E. Saltzmann, Christie Williams, Journal of Insect Physiology.

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User comments : 6

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Nemo
5 / 5 (1) Feb 08, 2010
As a Celiac I have to say Wheat is looking worse and worse as a food crop..
brant
not rated yet Feb 08, 2010
Are GMO foods OK?????? LOLOLOLOl

Wait till this gets into general population!!!!

Be free, spread with the winddd.....
Quantum_Conundrum
not rated yet Feb 08, 2010
Murphy's Law...

...so you engineer the wheat to activate these genes to kill the fly...in the process, 1% of humans become allergic to and/or death from wheat, AND some other bug decides Wheat tastes better, so it starts thriving on wheat because of the new genes...

"...a measure of wheat for a penny, and two measures of barley..."
sstritt
not rated yet Feb 09, 2010
Much more than 1% of the population has low level sensitivity to lectins in grains and legumes. See for yourself how much better you feel after elliminating them from your diet!
Caliban
1 / 5 (1) Feb 09, 2010
Yes- there is probably a link between the plant's defenses, and the several well-documented intolerance/sensitivity problems that humans have with digesting grains and legumes. And since there are also the same kind of problems noted for all foods for a small percentage of people, are we supposed to eat dirt? It's called co-evolution for a reason. Just have to keep an eye on the incidence and prevalence of reaction. None of these foods-UNALTERED- are likely to cause widespread harm, although you would expect to see greater numbers of sensitivity issues-merely as a function of population growth.
ormondotvos
not rated yet Feb 10, 2010
The committee for agricultural efficiency has reviewed your case and decided the good of the many overrides the pain of the few.

After all, humans are predators, to plants...

I suppose you really wanted that rainbow pony.

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