Nanoparticles may cause DNA damage across a cellular barrier

Nov 05, 2009
Nanoparticles may cause DNA damage across a cellular barrier

(PhysOrg.com) -- Scientists have shown in the laboratory that metal nanoparticles damaged the DNA in cells on the other side of a cellular barrier. The research, by the University of Bristol, is published online this week in Nature Nanotechnology.

Scientists have shown in the laboratory that damaged the DNA in on the other side of a cellular barrier. The nanoparticles did not cause the damage by passing through the barrier, but generated signalling molecules within the barrier cells that were then transmitted to cause damage in cells the other side of the barrier.
The research was carried out by a team at the University of Bristol and colleagues, and is published online this week in Nature .

The team grew a layer of human cells (about 3 cells in thickness) in the lab. They then used this barrier to examine the indirect effects of cobalt-chromium nanoparticles on cells that were lying behind this barrier.

The amount of DNA damage seen in the cells behind the protective barrier was similar to the DNA damage caused by direct exposure of the cells to the nanoparticles.

Dr Patrick Case, senior author on the study, said: “We need to be clear that our experimental set up is not a model of the human body. The cells receiving the exposure were bathed in culture media, whilst in the body they might be separated from the barrier by connective tissue and . The barrier cells were malignant cell line and 3 cells in thickness whilst all barriers in the body are less thick and of non .”

Gevdeep Bhabra, lead author on the paper, said: “Even though this work was done in the laboratory, our results suggest the existence of a mechanism by which biological effects can be signalled through a cellular barrier, thus it gives us insights into how barriers in the body such as the skin, the and the blood-brain barrier might work.”

Professor Ashley Blom, Head of Orthopaedic Surgery at the University of Bristol, added: “If barriers in the body do act in this way, then it gives us insight into how small particles such as metal debris or viruses may exert an influence in the body. It also highlights a potential mechanism whereby we might be able to deliver novel drug therapies in the future.”

These findings suggest that the indirect, as well as the direct, effects of nanoparticles on cells might be important when evaluating their safety.

More information: can cause across a cellular barrier, by Gevdeep Bhabra, Aman Sood, Brenton Fisher, Laura Cartwright, Margaret Saunders, William Howard Evans, Annmarie Surprenant, Gloria Lopez-Castejon, Stephen Mann, Sean A. Davis, Lauren A. Hails, Eileen Ingham, Paul Verkade, Jon Lane, Kate Heesom, Roger Newson and Charles Patrick Case. Nature Nanotechnology advance online publication on 5 November 2009.

Provided by University of Bristol (news : web)

Explore further: Thin diamond films provide new material for micro-machines

add to favorites email to friend print save as pdf

Related Stories

Nanoparticles may pose threat to liver cells, say scientists

Apr 04, 2006

Researchers at the University of Edinburgh are to study the effects of nanoparticles on the liver. In a UK first, the scientists will assess whether nanoparticles –already found in pollution from traffic exhaust, but also ...

Singapore nanotechnology combats fatal brain infections

Jun 28, 2009

Doctors may get a new arsenal for meningitis treatment and the war on drug-resistant bacteria and fungal infections with novel peptide nanoparticles developed by scientists at the Institute of Bioengineering and Nanotechnology ...

Engineering a 'Trojan horse' to sneak drugs into the brain

Sep 13, 2006

Beset by a host of debilitating and potentially fatal disorders, the human brain is in desperate need of a few good drugs. The catch, however, is that nature has set up a roadblock known as the blood-brain barrier — intended ...

DNA damage response confers a barrier for viral tumorigenesis

Sep 28, 2007

Kaposi’s sarcoma herpesvirus (KSHV) is a human tumor virus and an etiological agent for Kaposi’s sarcoma (KS). KSHV infection is endemic in sub-Saharan Africa where KS is nowadays the most common malignancy, due to widespread ...

Recommended for you

Light pulses control graphene's electrical behavior

5 hours ago

Graphene, an ultrathin form of carbon with exceptional electrical, optical, and mechanical properties, has become a focus of research on a variety of potential uses. Now researchers at MIT have found a way to control how ...

User comments : 1

Adjust slider to filter visible comments by rank

Display comments: newest first

Kedas
not rated yet Nov 06, 2009
I you play with small things then you play with small things (including DNA)
It is obvious that these kind of things would happen.