Better genetic markers developed for wheat streak mosaic virus resistance

Apr 29, 2014 by Kay Ledbetter
This wheat plant shows the signs of wheat streak mosaic virus. Credit: Dr. Shuyu Liu

(Phys.org) —Texas A&M AgriLife Research is developing genetic diagnostic markers to identify wheat streak mosaic virus resistance, providing wheat breeders a new tool in breeding wheat varieties resistant to one of the region's most prevalent diseases.

The study includes work by AgriLife Research scientists in Amarillo, including Dr. Shuyu Liu, small grains geneticist; Silvano Ocheya, doctoral graduate student in College Station; Dr. Jackie Rudd, breeder; and Dr. Qingwu Xue, crop stress physiologist.

They were joined by Dr. Guorong Zhang, wheat breeder at Kansas State University Agriculture Research Center at Hays, Kan.; and Dr. Guihua Bai, U.S. Department of Agriculture-Agricultural Research Service Central Small Grain Genotyping Center at Manhattan, Kan.

Wheat streak mosaic virus, which is transmitted by wheat curl mite, is one of the major limiting factors for wheat production in the Southern High Plains, Liu said. The yield loss can be up to 50 percent due to wheat streak mosaic virus in any given epidemic year.

There are several resistance genes to wheat streak mosaic available, but only one gene, labeled Wsm2, is from a wheat line, he said. The other two are from wheat relative species. Wsm2 has been bred in two wheat cultivars, RonL and Snowmass. This resistance gene is now being used by breeders in Kansas, Colorado and Texas to combine with other traits.

Ocheya, funded by Monsanto Beachell Borlaug Scholarship, is using a wheat population derived from CO960293-2 and TAM 111 to map the drought tolerance genes in TAM 111 and identify tight linked single nucleotide polymorphic or SNP markers, for drought tolerance genes and Wsm2. SNPs are variations within the DNA.

Zhang helped to evaluate the wheat streak infection on the population at growth chamber conditions under 64.5 degrees using virus strain Sidney 81.

The international wheat community, including the U.S., has developed a chip with 90,000 SNP markers, which can be used to screen wheat lines. Ocheya mapped about 5,000 SNP onto the whole wheat genome. Several tightly linked SNP markers to the Wsm2 gene were found, he said.

These markers were converted into high throughput SNP markers by Bai and his associates. These converted breeder-friendly SNP markers have been used to screen F2 breeding populations inoculated with the virus strain Sidney 81. This confirmed they are much better than any other previously used markers, Zhang said.

"These newly developed diagnostic SNP markers are being applied to marker-assisted selection for Wsm2 in several breeding programs in the High Plains, including Texas," Liu said.

Explore further: High yield, water efficiency of drought tolerant wheat due to higher biomass

add to favorites email to friend print save as pdf

Related Stories

Greenbug resistance gene found in wheat

Nov 27, 2013

Texas A&M AgriLife Research wheat scientists are getting closer to pinpointing the genes controlling greenbug resistance in wheat and recently published their findings.

Recommended for you

Parasitic worm genomes: largest-ever dataset released

5 hours ago

The largest collection of helminth genomic data ever assembled has been published in the new, open-access WormBase-ParaSite. Developed jointly by EMBL-EBI and the Wellcome Trust Sanger Institute, this new ...

Bitter food but good medicine from cucumber genetics

Nov 27, 2014

High-tech genomics and traditional Chinese medicine come together as researchers identify the genes responsible for the intense bitter taste of wild cucumbers. Taming this bitterness made cucumber, pumpkin ...

New button mushroom varieties need better protection

Nov 27, 2014

A working group has recently been formed to work on a better protection of button mushroom varieties. It's activities are firstly directed to generate consensus among the spawn/breeding companies to consider ...

Cataloguing 10 million human gut microbial genes

Nov 25, 2014

Over the past several years, research on bacteria in the digestive tract (gut microbiome) has confirmed the major role they play in our health. An international consortium, in which INRA participates, has developed the most ...

User comments : 0

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.