Researchers boost efficacy of drugs by using nanoparticles to target 'powerhouse of cells'

Sep 19, 2012
Shanta Dhar, right, an assistant professor of chemistry in the UGA Franklin College of Arts and Sciences, and doctoral student Sean Marrache have fabricated nanoparticles that boost the effectiveness of drugs by delivering them to the mitochondria of cells. Credit: John Paul Gallagher/University of Georgia

Nanoparticles have shown great promise in the targeted delivery of drugs to cells, but researchers at the University of Georgia have refined the drug delivery process further by using nanoparticles to deliver drugs to a specific organelle within cells.

By targeting mitochondria, often called "the powerhouse of cells," the researchers increased the effectiveness of mitochondria-acting therapeutics used to treat cancer, Alzheimer's disease and obesity in studies conducted with cultured cells.

"The is a complex organelle that is very difficult to reach, but these nanoparticles are engineered so that they do the right job in the right place," said senior author Shanta Dhar, an assistant professor of chemistry in the UGA Franklin College of Arts and Sciences.

Dhar and her co-author, doctoral student Sean Marrache, used a biodegradable, FDA-approved polymer to fabricate their nanoparticles and then used the particles to encapsulate and test drugs that treat a variety of conditions. Their results were published this week in early edition of the journal .

To test the effectiveness of their drug targeting system against cancer, they encapsulated the drug lonidamine, which works by inhibiting in the mitochondria, and, separately, a form of the antioxidant vitamin E. They then treated cultured and found that mitochondrial targeting increased the effectiveness of the drugs by more than 100 times when compared to the drugs alone and by five times when compared to the delivery of drugs with nanoparticles that target the outside of cells.

Similarly, the compound has shown promise in inhibiting formation of the that are a hallmark of Alzheimer's disease, but it quickly degrades in the presence of light and is broken down rapidly by the body. By encapsulating curcumin in the mitochondria-targeting nanoparticles, however, the researchers were able to restore the ability of brain cells in culture to survive despite the presence of a compound that encourages plaque formation. Nearly 100 percent of the cells treated with the mitochondria-targeting nanoparticles survived in the presence of the plaque-inducing compound, compared to 67 percent of cells treated with free curcumin and 70 percent of cells treated with nanoparticles that target the outside of cells.

Finally, the researchers encapsulated the obesity drug 2,4-DNP—which works by making energy production in the mitochondria less efficient—in their nanoparticles and found that it reduced the production of fat by known as preadipocytes by 67 percent compared to cells treated with the alone and by 61 percent of cells treated with nanoparticles that target the outside of cells.

"A lot of diseases are associated with dysfunctional mitochondria, but many of the drugs that act on the mitochondria can't get there," Marrache said. "Rather than try to alter the drugs, which can reduce their effectiveness, we encapsulate them in these nanoparticles and precisely deliver them to the mitochondria."

Dhar said that getting drugs to the mitochondria is no simple feat. Upon entering cells, nanoparticles enter a sorting center known as the endosome. The first thing Dhar and Marrache had to demonstrate was that the nanoparticles escape from the endosome and don't end up in the cells' disposal center, the lysosome.

The mitochondria itself is protected by two membranes separated by an interstitial space. The outer membrane only permits molecules of a certain size to pass through, while the inner membrane only permits molecules of a given range of charges to pass. The researchers constructed a library of nanoparticles and tested them until they identified the optimum size range—64 to 80 nanometers, or approximately 1,000 times finer than the width of a human hair—and an optimum surface charge, plus 34 millivolts.

Dhar notes the components they used to create the nanoparticles are FDA approved and that their methods are highly reproducible and therefore have the potential to be translated into clinical settings. The researchers are currently testing their targeted delivery system in rodents and say that preliminary results are promising.

"Mitochondrial dysfunctions cause many disorders in humans," Dhar said, " so there are several potential applications for this delivery system."

Explore further: Nano-scale gold particles are good candidates for drug delivery

Related Stories

Nanoparticles Improve Drug Targeting to Skin

Nov 27, 2006

Photodynamic therapy is a well-accepted treatment for a number of diseases of the skin, including several forms of skin cancer and actinic keratosis, a precancerous condition. However, the light-sensitizing agents used in ...

Gold used as safe driver of cancer drug

Mar 21, 2012

(PhysOrg.com) -- Gold nanoparticles can be used as delivery vehicles for platinum anticancer drugs, improving targeting and uptake into cells, according to research published in this month's edition of the ...

Watery Nanoparticles Deliver Anticancer Therapy

Mar 07, 2007

Ultrafine nanoparticles made of a lacy web of polymer and tiny pockets of water may prove to be an ideal vehicle for delivering light-activated drugs to tumors. Preliminary experiments, published in the journal Angewandte Ch ...

Recommended for you

User comments : 0