Pheromone increases foraging honey bees, leads to healthier hives

The study, which appeared this week in the journal, PLoS ONE, comes amid national concern over the existence of honey bee Colony Collapse Disorder (CCD) – a combination of events that result in the death of a bee colony. The causes behind CCD remain unknown, but researchers are focusing on four possible contributing factors: disease, pests, environmental conditions and nutrition.

According to Ramesh Sagili, coauthor on the study, "Division of labor associated with brood rearing in the honey bee: how does it translate to colony fitness?" resiliency to CCD may be increased through better hive management and the use of optimal dose of brood pheromone -- a chemical released by honey bee larvae that communicates the presence of larvae in the colony to adult bees. The optimal dose of brood pheromone that can stimulate colony growth may vary depending on the colony size, time of application and several other factors.

The number of larvae present in the hive affects the ratio of adult foraging bees to non-foragers in favor of foragers, said Sagili. In the study, when low levels of brood pheromone were introduced to experimental hives foragers collected more pollen, said Sagili.

Nectar is a carbohydrate source for both adults and larvae, while pollen is the primary source of protein. Nurse bees utilize pollen to produce brood food that is provisioned to the growing larvae in the colony. More pollen is equitable to better overall nutrition, one of the areas of concern in the appearance of CCD.

"The low brood pheromone treatment triggered higher pollen collection in the study," said Sagili, who holds an OSU Extension Service appointment and studies honey bee health, nutrition, pheromone biology and pollination in OSU's Department of Horticulture. "Colonies exposed to low levels of synthetic brood pheromone exhibited higher foraging populations, a decrease in the age of first foraging and greater foraging effort. The result is increased colony growth — an indicator of colony fitness."

The researchers also treated colonies with high levels of brood pheromone. They found the higher treatments were ineffective in increasing the number of foraging bees present in the colony, and in changing the amount of pollen an individual bee brought back to the hive.

"It's logical to assume that a higher dose of pheromone would result in higher pollen collection within the colonies as it would signify more larvae to rear," said Sagili. "Our results did not support that assumption."

An upper threshold for the pheromone may exist and when that threshold is reached a negative feedback might kick in, said Sagili. Colonies may try to balance the ratio of adults to larvae, and that in turn may lead to higher number of adults remaining in the nest for brood care and less bees foraging for resources.

Brood pheromone is not currently used in commercial bee keeping, however its application in research may result in the uncovering of mechanisms related to the division of labor, foraging strategies and colony fitness.

More information: The study is available online at http://dx.plos.org … pone.0016785

Provided by Oregon State University