Researchers reveal multifaceted regulation of crassulacean acid metabolism in epiphytic orchid

Epiphytes are a distinct group in the Earth's carbon cycling ecosystems. Most vascular epiphytes are from the particularly species-rich orchid family (Orchidaceae), with about 70% of Orchidaceae species being epiphytes. Crassulacean Acid Metabolism (CAM) is a water-conserving carbon dioxide (CO₂) fixation pathway, and Epiphytes with CAM photosynthesis are widespread in vascular plants.

By using a temporally separated carbon-concentrating mechanism, a CAM plant is able to photosynthesize during the day and exchange gases at night to minimize water loss. However, the understanding of the molecular regulation of CAM photosynthesis in epiphytes remains elusive.

Researchers from the Kunming Institute of Botany (KIB) of the Chinese Academy of Sciences (CAS) have assembled a high-quality genome of the epiphytic CAM orchid, Cymbidium mannii, integrating transcriptome, proteome and metabolome analysis to reveal the multifaceted regulation mechanism in CAM epiphytes.

Results have been published in Plant Communications entitled "High-quality Cymbidium mannii genome and multifaceted regulation of crassulacean acid metabolism in epiphytes."

According to the researchers, the genome of C. mannii is 2.88 Gb in length with a contig N50 of 22.7 Mb, of which 82.8% were repetitive elements. The genome size expansion in Cymbidium is mainly due to the long terminal repeats insertions, and the insertion time is consistent with the time of the diversification of Cymbidium species.

They found that the patterns of rhythmically oscillating metabolites, especially CAM-related products, reflect the circadian rhythmicity of metabolite accumulation in epiphytes.

Genome-wide analysis of transcript and protein level regulation revealed phase shifts in the multifaceted regulation of circadian metabolism. The researchers show the diurnal expression of several core CAM genes (in particular βCA and PPC), which could temporally fix carbon sources.

The gene expression levels of NADP-ME and PPDK in C. mannii were highly consistent with those in Kalanchoe fedtschenkoi and Sedum album, showing that they all prefer to use the nicotinamide adenine dinucleotide phosphate malic enzyme (NADP-ME) and pyruvate orthophosphate dikinase (PPDK) pathways for decarboxylation.

Since the circadian clock-associated genes and cis-regulatory elements (CREs) play a crucial role in regulating the CAM pathway, the researchers examined the expression pattern of the circadian clock and CAM genes during the diel cycle and found the light-responsive CREs in promoter regions that may be involved in photoperiodism.