Male fruit flies found to transfer chemical to females to induce sleep, so they won't mate with other males

Male fruit flies found to transfer chemical to females to induce sleep, so they won't mate with other males
Mating changes the onset of daily activity, masking a central function of the clock. This modulation is mediated by the sex peptide (transferred during copulation) acting on ppk+ neurons, which, in turn, directly contact pdf+ neurons, responsible for the increase of the activity that precedes dawn. Thus, our work identifies a post-mating response directly related to the circadian clock and begins to unravel the underlying neuronal circuit. Credit: Riva et al., 2022, PLOS Genetics, CC-BY 4.0 (creativecommons.org/licenses/by/4.0/)

A team of researchers from Bariloche Atomic Center and Fundación Instituto Leloir—IIBBA—CONICET, both in Argentina, has found that male fruit flies inject females with a chemical while mating that makes them sleep after sex so that they will not mate with other males.

In their paper published in the open access journal PLOS Genetics, the group describes how they studied reproduction of fruit flies in their lab using webcams.

Prior research has shown that during copulation, male fruit flies eject a peptide into the females' body along with a batch of sperm. Doing so has been shown to make the female less attractive to other males. Previous research has also shown that fruit flies in the wild wake up from a sleep state an hour or two before the sun comes up. In this new effort, the researchers looked to see if there was more to the story, from the male flies' perspective.

To find out, the researchers raised a group of in their lab and then added webcams that allowed them to monitor the activity among the flies for four days at a time.

Close Apposition of ppk+ and pdf+ Neurons. Confocal stacks showing overlap of arborizations between presynaptic terminals of ppk+ and pdf+ projection neurons in dorsal protocerebrum. The dendritic arbors and presynaptic terminals of ppk+ neurons were visualized by expression of the postsynaptic marker DenMark (orange) and the presynaptic marker syt-GFP (green), in a mated female brain. pdf neurons were visualized with anti-PDF (red). Credit: PLOS Genetics (2022). DOI: 10.1371/journal.pgen.1010258

From the video evidence, the researchers found that the only females that woke up and began to fly around in the morning, after the lights were turned on, were virgins. Suspecting that the peptide injected into the female during sex was behind the , the researchers repeated the experiment after first turning off the neurons in several of the females that responded to the peptide before freeing them to copulate.

The suspicions of the researchers were confirmed when the females with disabled neurons woke just before the lights came on along with the virgins. The researchers suggest this indicates that in addition to impacting how a female smells to other potential mates, the peptide also travels to the brain and interacts with the parts of the brain involved in sleep. They conclude by suggesting that the behavior of the males is a tactic that has evolved over time to help them ensure they are successful in producing offspring.

More information: Sabrina Riva et al, Mating disrupts morning anticipation in Drosophila melanogaster females, PLOS Genetics (2022). DOI: 10.1371/journal.pgen.1010258

Journal information: PLoS Genetics

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Citation: Male fruit flies found to transfer chemical to females to induce sleep, so they won't mate with other males (2022, December 23) retrieved 18 May 2024 from https://phys.org/news/2022-12-male-fruit-flies-chemical-females.html
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