Scientists propose reliable viral labeling strategy for visualization of EV71 infection

Scientists Propose Reliable Viral Labeling Strategy for Visualization of Ev71 Infection
Schematic illustration of bioorthogonal metabolic strategy based on protein biosynthesis for nonenveloped EV71 labeling and tracking. Credit: PAN Hong

Researchers led by Prof. Cai Lintao from the Shenzhen Institutes of Advanced Technology (SIAT) of the Chinese Academy of Sciences proposed a novel virus labeling strategy based on protein biosynthesis for dynamic visualization of nonenveloped enterovirus 71 infection. The study was published in ACS Applied Materials & Interfaces.

Enterovirus 71 (EV71), a major cause of severe hand-foot-and-mouth disease (HFMD), can develop into serious and life-threatening neurological complications, such as brainstem encephalitis, meningitis, and poliomyelitis-like paralysis. Understanding the viral invasion pathway of EV71 is important for elucidating its pathogenesis.

Although and chemical coupling have been applied to track viral infection in host cells and in vivo, these strategies could potentially interfere with viral invasive pathways.

The newly proposed strategy could be used for precisely dissecting the invasive pathway of EV71, especially for clarifying the process of viral RNA uncoating.

The researchers found that L-azidohomoalanine (Aha), an azido derivative of methionine, was more effective than azido sugars in introducing azido motifs into viral capsid proteins by substituting methionine residues during viral protein biosynthesis and assembly.

"The azide-modified EV71 particles are then effectively labeled with dibenzocyclooctyl-functionalized fluorescence probes through an in situ bioorthogonal reaction with well-preserved viral infectivity," said Prof. Cai.

"The dual-labeled imaging clearly clarifies that EV71 virions primarily bind to scavenger receptors and are internalized through clathrin-mediated endocytosis. The viral particles are then transported into early and late endosomes where viral RNA is released in a low-pH dependent manner at about 70 min post infection," He added.

The research is an important milestone in the study of enterovirus infection. It clearly showed clathrin-mediated endocytosis and uncoating processes of EV71, especially the spatio-temporal dynamics of the virus infection in host cells.

These findings may play an important role in revealing the invasion route and pathogenesis of EV71 .

More information: Fangfang Wang et al. Bioorthogonal Metabolic Labeling Utilizing Protein Biosynthesis for Dynamic Visualization of Nonenveloped Enterovirus 71 Infection, ACS Applied Materials & Interfaces (2019). DOI: 10.1021/acsami.9b17412

Citation: Scientists propose reliable viral labeling strategy for visualization of EV71 infection (2020, March 26) retrieved 12 April 2024 from
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.

Explore further

Novel mechanism for invasion of EV71 virus demonstrated


Feedback to editors