HSPC 'seeds' reveal VCAM-1+ macrophage role in homing process

November 21, 2018, Chinese Academy of Sciences
HSPCs preferentially stayed in the retention hotspots marked by red arrows. Credit: Prof. PAN Weijun's Group

Hematopoietic stem and progenitor cells (HSPCs) give rise to all blood lineages that support life. HSPCs, like seeds, need a suitable microenvironment to maintain their function. A process called "homing" allows HSPCs to anchor in their niches in order to expand and differentiate. Unique niche microenvironments composed of blood vessels and other niche components, including stromal cells, regulate this process.

To study the detailed architecture of the microenvironment and the regulation mechanism of homing, Prof. Pan Weijun's group at the Shanghai Institute of Nutrition and Health of Chinese Academy of Sciences used a zebrafish model to analyze the entire dynamic process of HSPC homing in vivo. The study, titled "VCAM-1+ macrophages guide the homing of HSPCs to a vascular niche," was published in Nature on Nov. 19, 2018.

By using a combination of advanced live imaging and a cell labeling-tracing system, researchers performed a high-resolution analysis of HSPC homing in zebrafish caudal hematopoietic tissue (CHT, equivalent to the fetal liver in mammals). Compared to itga4 mutants with homing defects, successful HSPC was defined in CHT as the lodgement of HSPCs for more than 30 minutes.

Schematic diagrams show how usher cells guide HSPCs into their vascular niche. Magenta cells: "usher cells"; VC: venous capillary; green lines: vessels. Credit: Prof. PAN Weijun's Group

The researchers also found that HSPCs preferred to stay at retention "hotspots" associated with venous capillaries, which are largely localized at the venous capillary confluence points connected to the caudal vein plexus. Further study showed that VCAM-1+ macrophages patrolling the inner surface of the venous plexus interact with HSPCs in an ITGA4-dependent manner and direct HSPC retention. These cells, named "usher cells," guide HSCP homing to two types of vascular niches. Usher cells, together with endothelial , help HSPC homing through distinct mechanisms.

This study dissects the temporal-spatial rules of HSPC retention, provides new insights into the mechanism for HSPC homing, and reveals the essential role of a VCAM-1+ macrophage population with patrolling behavior in HSPC retention.

Explore further: HSPC-derived CAR T-cells capable of lasting engraftment

More information: Dantong Li et al, VCAM-1+ macrophages guide the homing of HSPCs to a vascular niche, Nature (2018). DOI: 10.1038/s41586-018-0709-7

Related Stories

HSPC-derived CAR T-cells capable of lasting engraftment

January 3, 2018

(HealthDay)—Hematopoietic stem/progenitor cell (HSPC)-derived chimeric antigen receptor (CAR) T-cells are capable of long-term engraftment in a model of HIV/AIDS, according to a study published online Dec. 28 in PLOS Pathogens.

Bone marrow transplant stem cells can 'swim' upstream

January 4, 2018

When a cancer patient receives a bone marrow transplant, time is of the essence. Healthy stem cells, which can restart the production of blood cells and immune system components after a patient's own are compromised, need ...

Recommended for you

Coffee-based colloids for direct solar absorption

March 22, 2019

Solar energy is one of the most promising resources to help reduce fossil fuel consumption and mitigate greenhouse gas emissions to power a sustainable future. Devices presently in use to convert solar energy into thermal ...

EPA adviser is promoting harmful ideas, scientists say

March 22, 2019

The Trump administration's reliance on industry-funded environmental specialists is again coming under fire, this time by researchers who say that Louis Anthony "Tony" Cox Jr., who leads a key Environmental Protection Agency ...


Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.