Signs of bac­teria in the bovine fetus

September 5, 2018, University of Helsinki
Credit: CC0 Public Domain

Contrary to earlier assumptions, the intestines of newborn calves are not sterile, but contain DNA from various bacteria. Bacteria or their fragments originating in the mother may be significant to the development of the intestinal immune system.

Researchers at the University of Helsinki, Finland, have found bacterial DNA in the intestines of newborn calves, representing quite a diverse variety of bacterial species. Previously it was thought that the fetus develops in sterile conditions inside the uterus, delaying microbial colonisation until after the birth.

"Calves are an excellent study subject due to their conveniently large size already at birth. We are able to collect microbial samples directly from the rectum of newborn calves with sterile equipment doubly protected against contamination, making interference by microbes from the outside world impossible. In the case of human babies, researchers have to wait for them to defecate, which makes it very likely that bacteria already on the skin of the baby will contaminate the samples," explains Mikael Niku, a docent at the Faculty of Veterinary Medicine.

The early of resembles more the oral rather than faecal microbiota of adult cows.

"Similar results have been obtained also with . However, a fetal microbiota does not necessarily originate specifically in the mouth, but perhaps elsewhere at the beginning of the digestive tract, such as the rumen or the small intestine. Those are difficult areas from which to collect samples in living animals," says Mikael Niku.

In ruminants, the is of particular importance to the functionality of the immune system, since B cells, which produce antibodies, mature in the intestinal wall, whereas in humans this process takes place in the bone marrow. The research group led by Professor Antti Iivanainen at the Faculty of Veterinary Medicine found that in bovines, the intestinal immune system is already activated before birth.

After birth, the number of intestinal bacteria in calves quickly increases. Bacteria numbers were great already after the first day, but most of them were from the Escherichia genus. Within the first week after birth, the intestinal microbiota diversified and gradually started to gain the characteristics of adult faecal microbiota. However, the microbiota of young clearly indicated their primary source of nutrition: milk.

Modern DNA analysis comprehensively illustrates the composition of the microbiota. However, the presence of living in samples remains uncertain, since DNA can be preserved for some time also after microbes die. The research group is currently employing traditional bacterial culture to find out whether there are living microbes in the fetus.

"It would actually be enough for the maturation of the immune system for the dam to, as it were, provide readily processed microbial fragments to the fetus," muses Niku.

The group is studying the early microbiota of other domestic animals also, since the goal is to understand the phenomenon in mammals overall. Another objective is to find out whether the feed offered to dams can affect the microbiota of newborns, while also examining the development of the immune system.

"We mammals need species-specific intestinal microbiota originating in the dam or mother, as well as later on in life in other members of the same species. Our development takes place through interaction with these microscopic partners, enabling our immune system to identify friends and fight off harmful intruders. Determining the specifics of this dialogue is exceedingly interesting."

The researchers were helped in the collection of rectal samples by the staff of the cowshed at the Viikki Research Farm, University of Helsinki, and a group of energetic veterinary students monitoring the calving night and day.

"When calving was imminent, the students hurried to the cowshed to collect samples at the moment when the calf came out of the uterus. This is a fine example of how even students in the early stages of studies can take part in research," says Niku.

Explore further: How good bacteria can help keep a gut healthy

More information: Mohammad Jaber Alipour et al. The composition of the perinatal intestinal microbiota in cattle, Scientific Reports (2018). DOI: 10.1038/s41598-018-28733-y

Related Stories

How good bacteria can help keep a gut healthy

July 3, 2018

New research reveals a cellular mechanism by which good bacteria can help the gut stay healthy. The study, which appears in the journal Immunity, shows that good bacteria, or the microbiota, interact with both the epithelial ...

Mom may pass bacteria to baby in utero

July 6, 2018

For decades, it has been accepted that fetal development occurs in a sterile womb. Recent discoveries of bacterial DNA in baby's first bowel movement and in the placenta after sterile C-section, however, have challenged this ...

Gut bacteria affect ageing

April 19, 2017

It loses its pigments, its motor skills and mental faculties decline, it gets cancer – the turquoise killifish (Nothobranchius furzeri) struggles with the same signs of old age that affect many other living creatures. Researchers ...

Stress during pregnancy related to infant gut microbiota

January 26, 2015

Women who experience stress during pregnancy are likely to have babies with a poor mix of intestinal microbiota and with a higher incidence of intestinal problems and allergic reactions. This could be related to psychological ...

Recommended for you

How quinoa plants shed excess salt and thrive in saline soils

September 21, 2018

Barely heard of a couple of years ago, quinoa today is common on European supermarket shelves. The hardy plant thrives even in saline soils. Researchers from the University of Würzburg have now determined how the plant gets ...

Basking sharks can jump as high and as fast as great whites

September 20, 2018

A collaborative team of marine biologists has discovered that basking sharks, hundreds of which are found off the shores of Ireland, Cornwall, the Isle of Man and Scotland, can jump as fast and as high out of the water as ...

Decoding the structure of an RNA-based CRISPR system

September 20, 2018

Over the past several years, CRISPR-Cas9 has moved beyond the lab bench and into the public zeitgeist. This gene-editing tool CRISPR-Cas9 holds promise for correcting defects inside individual cells and potentially healing ...

0 comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.