How green algae assemble their enzymes

How green algae assemble their enzymes
Anne Sawyer has gained new insights into the protein machinery of green algae. Credit: RUB, Kramer

Researchers at Ruhr-Universität Bochum have analysed how green algae manufacture complex components of a hydrogen-producing enzyme. The enzyme, known as the hydrogenase, may be relevant for the biotechnological production of hydrogen.

To date, little is known about the way organisms form this type of hydrogenases under natural conditions. Using novel synthetic biology methods, the team around Dr Anne Sawyer, PhD student Yu Bai, assistant professor Dr Anja Hemschemeier and Prof Dr Thomas Happe from the Bochum-based research group Photobiotechnology, discovered that a specific protein machinery in the green algal chloroplasts is required for the production of a functional hydrogenase. The researchers published their findings in The Plant Journal.

Complex structure

The team worked with the single-cell alga Chlamydomonas reinhardtii. These organisms have a specific protein machinery in different regions of the cells that assembles enzymes – e.g. in the photosynthesis-conducting chloroplasts and in the cell fluid, i.e. the cytoplasm.

One that requires such assembly is the HYDA1 enzyme, which contains a complex cofactor, which is the area inside the enzyme where the actual hydrogen production takes place. The cofactor consists of a cluster of four iron and four sulphur atoms; a configuration frequently found in enzymes. What is unusual, however, is that a second cluster of two additional iron atoms binds to it for the hydrogen catalysis.

How green algae assemble their enzymes
Thomas Happe and Anne Sawyer. Credit: RUB, Kramer

Special protein machinery necessary

Happe, Sawyer and their colleagues intended to identify the elements necessary for producing the cofactor in the living cell. They introduced hydrogenase precursors in different regions of the green algal cell, namely in the and the cytoplasm. The in the chloroplast was the only one capable of assembling a functioning hydrogenase. The machinery in the cytoplasm couldn't produce the complex cofactor.

Bacterial enzyme in green algae

In a subsequent test, the researchers implanted the blueprint of a bacterial hydrogenase in the green algal genome. Chlamydomonas reinhardtii used it to produce a functional enzyme that efficiently generated hydrogen.

"Based on these findings, we can develop biotechnological methods, in order to achieve efficient hydrogen production in ," says Happe. "We now know that the that assembles enzymes in the chloroplasts is unique and irreplaceable."


Explore further

Hydrogen synthesis—when enzymes assemble themselves in the test tube

More information: Anne Sawyer et al. Compartmentalisation of [FeFe]-hydrogenase maturation in, The Plant Journal (2017). DOI: 10.1111/tpj.13535
Journal information: The Plant Journal

Citation: How green algae assemble their enzymes (2017, March 27) retrieved 19 September 2019 from https://phys.org/news/2017-03-green-algae-enzymes.html
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.
6 shares

Feedback to editors

User comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more