High-throughput screening strategy identifies compounds active against antibiotic-resistant bacteria

May 11, 2016, Mary Ann Liebert, Inc
Credit: ©Mary Ann Liebert, Inc., publishers

A new study in which researchers rapidly screened more than 11,000 bioactive molecules for activity against an antibiotic-resistant strain of Klebsiella pneumoniae bacteria identified multiple compounds with potent antimicrobial activity. These active compounds included two existing drugs, azidothymidine, an antiviral used to treat HIV infection, and spectinomycin, an antibacterial agent used to treat gonorrhea, as reported in ASSAY and Drug Development Technologies.

Kenneth Smith and James Kirby, Beth Israel Deaconess Medical Center, Boston, MA, describe a high-throughput screening (HTS) assay they developed that is capable of detecting either the direct antibiotic activity of small molecules or their ability to act as adjunctive treatments by synergistically restoring the effectiveness of an antibiotic drug to which bacteria have developed resistance.

The article "Validation of a High-Throughput Screening Assay for Identification of Adjunctive and Directly Acting Antimicrobials Targeting Carbapenem-Resistant Enterobacteriaceae" describes how HTS of large compound libraries could lead to the identification of more effective therapies against intractable and potentially life-threatening infections.

"The paper by Smith and Kirby exemplifies the importance of a carefully designed strategy for HTS and follow-up characterization of active ," says ASSAY and Drug Development Technologies Editor-in-Chief Andrew D. Napper, PhD, Associate Director, Nemours Center for Childhood Cancer Research, Head - High Throughput Screening/Drug Discovery Laboratory, and Senior Research Scientist, Nemours/A.I. duPont Hospital for Children (Wilmington, DE). "By implementing this strategy, these authors efficiently and unequivocally identified compounds with clinically important anti-bacterial activity."

Explore further: Screening method uncovers drugs that may combat deadly antibiotic-resistant bacteria

More information: Kenneth P. Smith et al, Validation of a High-Throughput Screening Assay for Identification of Adjunctive and Directly Acting Antimicrobials Targeting Carbapenem-Resistant Enterobacteriaceae, ASSAY and Drug Development Technologies (2016). DOI: 10.1089/adt.2016.701

Related Stories

New compounds have potential to combat Lyme disease

April 11, 2016

When physicians diagnose Lyme disease, they usually prescribe standard antibiotics—and for many patients, that's enough. But for 10 to 20 percent of patients, the disease persists, causing joint paint, neurological difficulties ...

Recommended for you

The powerful meteor that no one saw (except satellites)

March 19, 2019

At precisely 11:48 am on December 18, 2018, a large space rock heading straight for Earth at a speed of 19 miles per second exploded into a vast ball of fire as it entered the atmosphere, 15.9 miles above the Bering Sea.

Revealing the rules behind virus scaffold construction

March 19, 2019

A team of researchers including Northwestern Engineering faculty has expanded the understanding of how virus shells self-assemble, an important step toward developing techniques that use viruses as vehicles to deliver targeted ...

OSIRIS-REx reveals asteroid Bennu has big surprises

March 19, 2019

A NASA spacecraft that will return a sample of a near-Earth asteroid named Bennu to Earth in 2023 made the first-ever close-up observations of particle plumes erupting from an asteroid's surface. Bennu also revealed itself ...

Nanoscale Lamb wave-driven motors in nonliquid environments

March 19, 2019

Light driven movement is challenging in nonliquid environments as micro-sized objects can experience strong dry adhesion to contact surfaces and resist movement. In a recent study, Jinsheng Lu and co-workers at the College ...

0 comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.