Discovery of mechanism that enables bacteria to elude antibiotics

January 29, 2016, Universitat Autonoma de Barcelona
Model of the behavior of a bacterial colony of Salmonella enterica due to the presence of antibiotics. Credit: UAB

Researchers from UAB describe for the first time, in a work published in PLOS ONE, a model of the behaviour of a bacterial colony that shows how it protects itself against toxic substances like antibiotics during the colonisation process.

The Molecular Microbiology Research Group in the UAB's Department of Genetics and Microbiology has determined that alteration of the equilibrium between two proteins of Salmonella enterica in the presence of leads to the disorganisation of the structures that allow the population to spread, which in turn stops the progress of the cells in the that are nearest to harmful concentrations of antibiotic, while the rest spread into areas with lower concentrations.

Bacterial populations move over surfaces in coordinated way known as swarming, which allows them to spread further over organs and tissues and increases the virulence of the infection. This movement is driven by the action of the flagella and the chemoreceptors, the systems responsible for identifying chemical compounds in the environment and which are anchored at the poles of their cells, forming highly organised structures, of which the protein CheW forms part.

The researchers have shown that activation of the SOS system, a cellular response in the bacteria in the presence of antibiotics, causes an increase in the concentration of the protein RecA, which interferes with the distribution of the CheW protein by altering the organisation of the chemoreceptors and stopping the swarming movement.

Fluorescence microscope image of the polar chemoreceptor clusters in Salmonella enterica. Credit: UAB

The imbalance between the concentrations of these two proteins makes the bacterial colony avoid any areas of the surface they are colonising that present harmful concentrations of antibiotic, stopping the swarming movement in the regions nearest to the drug and allowing the colonization of the rest of the area.

The molecular balance between the RecA and CheW proteins is therefore seen to be crucial to the organisation of the chemoreceptors in the cells of the bacteria and so to their colonising movement.

This work also shows that if the dose of antibiotic in that area is reduced to non-harmful levels, the drop in the concentration of RecA and the re-establishment of equilibrium with CheW allows the chemoreceptors to be structured once more, restoring the swarming movement and, as a result, the colonisation of that area.

In the words of the researchers, the results show clearly that the bacterial populations move over the surfaces using specific mechanisms like that described in this work, in order to avoid contact with the compounds that damage their DNA.

Salmonella enterica is member of a bacterial group that includes several pathogenic species responsible for diseases in the digestive and respiratory system, such as septicaemia and systemic infections.

The work opens the door to the design of new compounds that can neutralise this bacterial strategy, which reduces the efficiency of treatment with antibiotics.

Explore further: Discovery of controlled swarm in bacteria

More information: Oihane Irazoki et al. SOS System Induction Inhibits the Assembly of Chemoreceptor Signaling Clusters in Salmonella enterica, PLOS ONE (2016). DOI: 10.1371/journal.pone.0146685

Related Stories

Discovery of controlled swarm in bacteria

June 22, 2010

A study led by Spanish researchers from Universitat Autonoma de Barcelona (UAB) describes one of the mechanisms in which pathogenic bacteria populations control the way they spread over the surface of the organs they infect ...

Antibiotic resistance is a gut reaction

December 16, 2014

Scientists from the Institute of Food Research and the University of East Anglia have discovered how certain gut bacteria can protect themselves and others in the gut from antibiotics.

Strategies to decrease bacterial colonization

September 14, 2015

Among the bacterial infections that are most difficult to treat, chronic infections associated with bacterial biofilms are one of the most hazardous. Bacterial biofilms are densely packed communities of microbial cells surrounded ...

Recommended for you

Scale-eating fish adopt clever parasitic methods to survive

January 17, 2018

Think of them as extra-large parasites. A small group of fishes—possibly the world's cleverest carnivorous grazers—feeds on the scales of other fish in the tropics. The different species' approach differs: some ram their ...

How living systems compute solutions to problems

January 17, 2018

How do decisions get made in the natural world? One possibility is that the individuals or components in biological systems collectively compute solutions to challenges they face in their environments. Consider that fish ...


Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.