Investigators insert large DNA sequence into mammalian cells

For the first time, researchers have used a simplified technique derived from a defense mechanism evolved by bacteria and other single-celled organisms to successfully insert a large DNA sequence into a predetermined genomic site in mammalian cells.

The methods used may help investigators genetically engineer cells to produce high levels of certain proteins—for example by placing the DNA sequence of a particular protein at the site of a highly active gene.

"The CRISPR-Cas system has been previously used to insert a foreign DNA sequence into a targeted genomic site in via a process of recombination. Here we showed that the insertion could be performed using a simplified end joining process," said Dr. Lawrence Chasin, senior author of the Biotechnology and Bioengineering study. "This simplification may prove especially useful for high throughput targeting approaches."


Explore further

Unlocking the key to immunological memory in bacteria

More information: DOI: 10.1002/bit.25629
Journal information: Biotechnology and Bioengineering

Provided by Wiley
Citation: Investigators insert large DNA sequence into mammalian cells (2015, July 6) retrieved 25 May 2019 from https://phys.org/news/2015-07-insert-large-dna-sequence-mammalian.html
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.
24 shares

Feedback to editors

User comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more