Fecal transplants let packrats eat poison

July 21, 2014, University of Utah
Fecal transplants let packrats eat poison
A captive desert woodrat, also known as a packrat, stands on a rock near branches from a toxic creosote bush. Credit: Kevin Kohl, University of Utah.

Woodrats lost their ability to eat toxic creosote bushes after antibiotics killed their gut microbes. Woodrats that never ate the plants were able to do so after receiving fecal transplants with microbes from creosote-eaters, University of Utah biologists found.

The new study confirms what biologists long have suspected: bacteria in the gut – and not just liver enzymes – are "crucial in allowing herbivores to feed on ," says biologist Kevin Kohl, a postdoctoral researcher and first author of the paper published online today in the journal Ecology Letters.

The study of woodrats, also known as packrats, raises two concerns, according to Kohl and the study's senior author, Denise Dearing, a professor and chair of biology:

  • Endangered species may lose diversity of their gut microbes when they are bred in captivity. When they are released to the wild, does that leave them unable to consume toxic plants that once were on their menu?
  • Livestock like cows often are fed antibiotics to promote growth. Does that impair their ability to eat toxic plants like locoweed when drought reduces pasture grass?

The study of woodrats someday might impact farming practices in arid regions, where toxic plants like creosote and juniper are abundant. Livestock now can't graze on these cheap food sources. Could interspecies transplants of gut microbes help livestock expand their dining menu? Kohl says he'd like to transplant woodrat gut microbes into sheep or goats to find out if that increases their tolerance to toxic foods.

"Juniper is expanding its range, and ecologists and land managers are concerned," he says. "Farmers are interested in getting their sheep and goats to eat juniper."

Evolving a Taste for Toxins

Many plants produce toxic chemicals, which they use as a defense against herbivores, or plant-eating animals. A toxic resin coats the leaves of the creosote bush; juniper toxins are found inside juniper needles.

Most mammals are herbivores. Some face serious challenges: their bodies must handle up to hundreds of toxic chemicals from the plants they consume each day. "Plant toxins determine which plants a herbivore can eat," says Kohl.

Liver enzymes help animals detoxify such poisons. Researchers previously isolated toxin-degrading microbes from herbivores, but Kohl and Dearing say that, until now, scientists have lacked strong evidence for what has been conventional wisdom: Gut microbes also help some herbivores eat toxic plants.

The study involved desert woodrats (Neotoma lepida) – grayish rodents native to western North American deserts. Woodrats somehow acquired novel toxin-degrading gut microbes to adapt to climate and vegetation changes that began 17,000 years ago.

"In a natural climatic event at the end of the last glacial period, the Southwest dried out and our major deserts were formed," Dearing says. Creosote, which was native to Mexico, moved north into the Mojave Desert and replaced juniper there, but did not go farther north into Great Basin deserts. Desert woodrats in the Mojave started eating creosote bushes, while desert woodrats in the Great Basin kept eating toxic juniper, to which they had adapted earlier.

At first, the ancient juniper eaters in the Mojave likely were poorly equipped to eat invading creosote, but scientists believe microbes sped up their dietary adjustment. Though slow, evolutionary genetic changes in herbivores play an important role in adapting to new diets. "Transfer of toxin-degrading microbes from one organism to the other is much more rapid," Dearing says.

How do woodrats get their tiny, but valuable bacterial helpers today?

"Mammals acquire microbes during birth, through contact with their mother's vaginal and fecal microbes," Kohl says. "Other possible places to get microbes include leaf surfaces, the soil or feces that woodrats collect from other animals."

Speeding Up Dietary Evolution with Fecal Transplants

In an earlier study, the Utah researchers showed that the creosote-eating woodrats from the Sonoran and Mojave deserts had higher proportions of gut microbes that might detoxify creosote, while juniper-eating woodrats from the Great Basin had a different set of gut bacteria.

In the new study, Dearing and colleagues performed three experiments using two kinds of woodrats—juniper eaters from the Great Basin desert and creosote eaters from the Mojave Desert. They were captured and kept in the lab on a diet of rabbit chow.

In the first experiment, the scientists studied the relative abundances of gut-microbe genes in two groups of the creosote-eating Mojave woodrats. One group was fed rabbit chow containing 1 percent of creosote resin for two days, followed by rabbit chow with 2 percent of creosote resin for three days. The control group was fed only rabbit chow. Gut microbes were removed from the foreguts of both woodrat groups. DNA was isolated from the microbes to identify genes involved in detoxification.

The scientists found that a woodrat's diet determines the composition of its gut microbes. "Mammals are adapted to the plant toxins they eat," Kohl says. The guts of creosote-fed woodrats were teeming with microbes that may degrade creosote, while the guts of creosote-free woodrats had only one-fourth the levels of the same gut microbes.

In the second experiment, the researchers experimentally removed gut microbes to highlight their dietary role in woodrats. Antibiotics kill about 90 percent of the gut microbes in animals, severely impairing their ability to consume toxic foods.

Two groups of woodrats were pretreated with the antibiotic neomycin in their drinking water. One group was placed on a diet of rabbit chow and creosote resin. With their gut microbes killed by the antibiotic, they were unable to feed on creosote and lost 10 percent of their body weight within 13 days. The second group ate only rabbit chow and didn't lose weight, showing that killing their gut microbes didn't harm them because they weren't eating toxic creosote.

In the third experiment, the biologists essentially sped up evolution by using fecal transplants to quickly change populations of microbes living in the woodrats' guts. They conducted fecal transplants and showed that acquiring new microbes indeed helped woodrats adopt new diets.

Woodrats naturally eat their own and other woodrats' feces. So in the experiment, juniper-eating Great Basin woodrats were fed rabbit chow mixed with feces either from other juniper eaters or from creosote-eating Mojave woodrats. Both woodrat groups then were challenged with a creosote diet.

After ingesting feces – and thus gut microbes – from creosote eaters, juniper eaters persisted for 11 days on the creosote diet without losing much weight. Yet 65 percent of the juniper eaters that ate feces of other juniper eaters didn't gain microbes that detoxify creosote, so they lost 10 percent of their weight by day 11 on a creosote diet.

It's not that those woodrats rejected creosote-laced food. They ate as much as the woodrats that were fed feces with creosote-detoxifying microbes. Instead, Kohl and co-authors found that when woodrats didn't get transplants of creosote-detoxifying microbes, their urine was more acidic, suggesting their livers expended a lot of energy to degrade creosote toxins.

But in juniper eaters that consumed the feces of creosote eaters, their newly acquired likely detoxified most of the , taking the burden off of liver enzymes.

Explore further: Poison: It's what's for dinner

Related Stories

Poison: It's what's for dinner

April 6, 2009

As the U.S. Southwest grew warmer from 18,700 to 10,000 years ago, juniper trees vanished from what is now the Mojave Desert, robbing packrats of their favorite food. Now, University of Utah biologists have narrowed the hunt ...

Rats control appetite for poison

August 9, 2011

(PhysOrg.com) -- Life is tough for woodrats in deserts of the U.S. Southwest. There are few plants for food, and those plants produce poison to deter rodents, insects and other animals. A new University of Utah study shows ...

Team develops method of identifying impact of gut microbes

January 23, 2014

(Medical Xpress)—A team of researchers at Washington School of Medicine in St. Lois has developed a method for identifying the impact that individual strains of microbes in the human gut have on the person housing them. ...

Gut microbes turn carbs into colorectal cancer

July 17, 2014

Colorectal cancer has been linked to carbohydrate-rich western diets, but the underlying mechanisms have been unclear. A study published by Cell Press July 17th in the journal Cell shows that gut microbes metabolize carbohydrates ...

Variety in diet can hamper microbial diversity in the gut

May 28, 2014

Scientists from The University of Texas at Austin and five other institutions have discovered that the more diverse the diet of a fish, the less diverse are the microbes living in its gut. If the effect is confirmed in humans, ...

Recommended for you

Scientists shed light on biological roots of individuality

February 16, 2018

Put 50 newborn worms in 50 separate containers, and they'll all start looking for food at roughly the same time. Like members of other species, microscopic C. elegans roundworms tend to act like other individuals their own ...

Plants are given a new family tree

February 16, 2018

A new genealogy of plant evolution, led by researchers at the University of Bristol, shows that the first plants to conquer land were a complex species, challenging long-held assumptions about plant evolution.


Adjust slider to filter visible comments by rank

Display comments: newest first

not rated yet Jul 21, 2014
We humans also have developed an inability to keep our yeast in its Dr Jeckyl form; Mr Hyde is responsible for the seemingly unlimited effects of fungal candida. i wonder if the fecal bacteria transplant that works to restore gut bacteria would help the thousands of gas bloated agonizers desperate for relief.
not rated yet Jul 21, 2014
Add 'detoxification' to the numerous necessary functions performed by our enteric commensals. The 1000+ species of bacteria/fungi/yeast that can thrive within us appear to be able to protect us from some toxins, infections, inflammatory diseases (arthritis, rheumatism, Chrone's disease, hypertension, etc.) and even some cancers.
not rated yet Jul 22, 2014
"The procedure, which involves transplanting a healthy fecal sample from a donor into the GI tract of a patient suffering from recurrent C. difficile infections, is gaining popularity, but Dr. Brasco says the idea of transplanting poop has yet to be completely accepted. "You wouldn't have even thought about doing this a year and a half or two years ago," he says. "More and more people are doing it now.""

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.