Nanopores light up for reading out DNA

September 12, 2013, Delft University of Technology
Nanopores light up for reading out DNA
Artist impression of a nanopore with Plasmonic antennas.

Nanopores are ideally suited for threading DNA molecules through them, enabling the genetic code to be read out. Researchers from TU Delft want to make this technology even more powerful by equipping the pores with 'plasmonics'. By utilising minuscule optical 'antennas', it is possible to focus light precisely and intensely on the nanopore. Eventually, the researchers hope to use this technique to control DNA and read it out efficiently.

Reduced costs

The costs for producing a readout of the (our DNA) have fallen significantly in the past decade, but the technology has remained relatively expensive. Nanopores in are a suitable candidate for a new generation of DNA sequencers. A decade ago, research by scientists from TU Delft and Harvard University was at the forefront of this technology.

Optical 'antennas'

Together with colleagues from the University of Illinois (USA), the TU Delft scientists now want to go a step further: they are equipping the nanopores with 'plasmonics', minuscule optical 'antennas'. These are used to focus light to an highly intense and extremely small 'hot spot' in the nanopore. In doing so, the researchers hope to be able to capture, slow down and read out the DNA efficiently.

The research is being funded by the American National Institute of Health (NIH) to the amount of 2.47 million dollars. It is part of a cluster of eight funded research projects worth 17 million dollars, as announced this week by the NIH.

Sensitive sensor

"Reading the base pairs in DNA with current sequencing technology is expensive. You need to label the DNA and only very short pieces of DNA can be read out, approximately a few hundred ," says professor Cees Dekker, head of the research project and director of the Kavli Institute of Nanoscience at TU Delft. "Nanopores offer the possibility of reading out very long strands of DNA in a single go. By equipping the nanopores with plasmonics, we hope to integrate a new ultrasensitive type of sensor precisely on the spot of the nanopore."

Nanopores light up for reading out DNA
Lycurgus Cup. Credit: Brittish Museum

A thousand times stronger

Dekker's research group and the colleagues in Illinois are the first to combine both fields of study. "We make minuscule plasmonic antennas: metal structures on which you can focus electrons with the help of light. By placing these tiny antennas around the , we're able to 'light up' the pore. At precisely this spot, the light intensity is focused a thousand times stronger. And in doing so, we think we can control the DNA molecule and at the same time read it out," explains post-doc Magnus Jonsson.

Roman art

Research in both the fields of nanopores and plasmonics is evolving rapidly. Plasmonics is a relatively recent field of study, but according to Dekker the use of plasmonic materials has been around for years. "Roman artists in the fourth century were already using gold and silver dust in glass. A fine example of this is the Lycurgus Cup, which is either red or green depending on the fall of light. This is also plasmonics. The effect has therefore been used for millennia, even though we only found out recently how it works."

Explore further: DNA through graphene nanopores

Related Stories

DNA through graphene nanopores

July 12, 2010

A team of researchers from Delft University of Technology (The Netherlands) announces a new type of nanopore devices that may significantly impact the way we screen DNA molecules, for example to read off their sequence. In ...

New system to improve DNA sequencing

April 3, 2013

( —A sensing system developed at Cambridge is being commercialised in the UK for use in rapid, low-cost DNA sequencing, which would make the prediction and diagnosis of disease more efficient, and individualised ...

Research: Hopping DNA supercoils

September 14, 2012

If you take hold of a DNA molecule and twist it, this creates 'supercoils', which are a bit like those annoying loops and twists you get in earphone cables. Research carried out by TU Delft, The Netherlands, has found that ...

Advance in nanotech gene sequencing technique

May 20, 2013

( —The allure of personalized medicine has made new, more efficient ways of sequencing genes a top research priority. One promising technique involves reading DNA bases using changes in electrical current as they ...

Recommended for you

A soft solution to the hard problem of energy storage

May 18, 2018

It's great in the lab, but will it actually work? That's the million-dollar question perpetually leveled at engineering researchers. For a family of layered nanomaterials, developed and studied at Drexel University—and ...

New blood test rapidly detects signs of pancreatic cancer

May 17, 2018

Pancreatic cancer is expected to become the second deadliest cancer in the United States by 2030. It is tough to cure because it is usually not discovered until it has reached an advanced stage. But a new diagnostic test ...

Metallic drivers of Alzheimer's disease

May 17, 2018

X-ray spectromicroscopy at the Scanning X-ray Microscopy beamline (I08), here at Diamond, has been utilised to pinpoint chemically reduced iron and calcium compounds within protein plaques derived from brains of Alzheimer's ...


Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.