Drug researchers hunting for alternative ways to treat human immunodeficiency virus (HIV) infections may soon have a novel targetits camouflage coat. HIV hides inside a cloak unusually rich in a sugar called mannose, which it uses to slip past the immune system before infecting its hosts cells. Recently, however, biochemists discovered a family of chemical compounds that stick strongly to mannose. Understanding how this mechanism works could reveal a way to make drugs adhere to and kill HIV. Yu Nakagawa and Yukishige Ito at the RIKEN Advanced Science Institute in Wako and their colleagues from several research institutes in Japan are leading the effort: they have mapped the binding site of the mannose-binding compound pradimicin A.
Mannose-binding compounds are particularly attractive to drug researchers thanks to their double-action anti-HIV effect. By sticking to mannose in the viruss coat, pradimicin A first freezes HIVs molecular machinery for entering and infecting its hosts healthy cells. The virus responds by reducing the mannose in its coat thereby revealing itself to the immune system, which can then attack.
Unraveling just how pradimicin A recognizes mannose, however, has proven surprisingly difficult. In solution, the two components stick together in variously sized small clusters, confounding conventional analytical techniques such as solution-based nuclear magnetic resonance (NMR) and x-ray crystallography. Nakagawa, Ito and their colleagues side-stepped the clumping problem by using solid-state NMR, which allowed them analyze the compounds as solids, rather than in solution.
The research teams approach involved inserting carbon-13, a chemical label, into particular parts of the pradimicin A structure. Carbon-13 boosts the NMR signal wherever it is inserted, so the team could walk around the compound and detect where it interacts most strongly with mannose.
The results revealed that pradimicin A curls up to form a cavity, within which the mannose structure sits (Fig. 1). Our study highlights the benefit of solid-state NMR methodology to investigate this interaction, says Nakagawa. Solid-state NMR is, at present, the only technique to analyze such a complicated system. Flagging the potential utility of the technique, Nakagawa adds that: Our analytical strategy might be applicable to other systems that similarly suffer from aggregation in solution.
Meanwhile, solid-state NMR can offer even more in probing mannosepradimicin A binding, Nakagawa says. Having determined how and where pradimicin A grabs mannose, the teams next step will be to use the technique to identify the specific molecular interactions that bind the pradmicin A to this potential Achilles' heel of HIV.
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Nakagawa, Y., et al. Mapping of the primary mannose binding site of pradimicin A. Journal of the American Chemical Society 133, 1748517493 (2011).