Researchers discover proton diode

Sep 02, 2010
This is the proton diode in the light-driven proton pump bacteriorhodopsin. Credit: RUB

Biophysicists in Bochum have discovered a diode for protons: just like the electronic component determines the direction of flow of electric current, the "proton diode" ensures that protons can only pass through a cell membrane in one direction. Water molecules play an important role here as active components of the diode.

The researchers led by Prof. Dr. Klaus Gerwert (Chair of Biophysics at the RUB) were able to observe this through a combination of , X-ray crystallography, time-resolved FTIR spectroscopy and biomolecular simulations. They report in the current international online edition of the journal Angewandte Chemie.

Protons drive the protein turbines

The proton diode plays an important role in the energy production of cells. Light-driven proton pumps - certain proteins that traverse the - eject protons out of the cell, so that excess pressure is generated outside "much like the water pressure at a dam", explains Prof. Gerwert. Elsewhere, the protons push back into the cells to compensate the concentration gradient, and thereby drive the turbines of the cell, proteins known as ATPases. The energy thus released is converted into the universal fuel of the cells, ATP (adenosine triphosphate). "This process is a kind of archaic photosynthesis" explains Prof. Gerwert. "The light energy is ultimately converted into usable energy for the organism".

We used to believe in chance

The details of these processes are the subject of research. In particular, the role of water molecules in proteins has long been unclear. "Previously it was believed that the water molecules blundered into the proteins by chance, and fulfilled no particular function", says Gerwert. Manfred Eigen, born in Bochum in 1967, was awarded the Nobel Prize for chemistry because he was able to explain why water and ice are such rapid conductors. The current work shows that proteins also use precisely this mechanism and that the water molecules do indeed carry out an active function in the protein.

Water is as important as amino acids

This result supports the hypothesis drawn up by Klaus Gerwert in 2006 in Nature that protein-bound are just as important catalytic elements for the function of proteins as amino acids, the building blocks of life. Consequently, the Bochum biophysicists have devoted their work in Angewandte Chemie to Manfred Eigen. Eigen also published his central thesis on proton transfer in water in in 1964. Klaus Gerwert was inspired by Manfred Eigen's winter seminars in Klosters.

Film instead of fixed image

The Bochum researchers were able to achieve their results in an interdisciplinary approach through a combination of molecular biology, X-ray crystallography, time-resolved FTIR spectroscopy and biomolecular simulations. This combination shows the dynamic processes in the protein after light excitation with atomic resolution. "You can track how the proton is transported from the central proton binding site inside the via an amino acid and then via a protonated water cluster to the membrane surface", says Prof. Gerwert. The interdisciplinary approach is now expanding the classical methods of structural biology, X-ray crystallography and nuclear magnetic resonance spectroscopy (NMR), as it provides a complete film and not just fixed images of proteins. The experiments in Bochum were supplemented by computer simulations in Shanghai. Klaus Gerwert is both a professor at the RUB and Director of the Max Planck Partner Institute for Computational Biology in Shanghai.

Explore further: A new approach to creating organic zeolites

More information:
-- Wolf, S., Freier, E., Potschies, M., Hofmann, E. and Gerwert, K.: "Directional Proton Transfer in Membrane Proteins Achieved through Protonated Protein-Bound Water Molecules: A Proton Diode" Angewandte Chemie International Edition, DOI: 10.1002/anie.201001243

-- Garczarek, F., Gerwert, K.: "Functional waters in intraprotein proton transfer monitored by FTIR difference spectroscopy". In: Nature 439, 109-112 (2006)

Provided by Ruhr-University Bochum

4 /5 (2 votes)
add to favorites email to friend print save as pdf

Related Stories

Identifying the 'Signatures' of Protons in Water

Jul 13, 2005

Free protons from acids associate with 1, 2 or 3 molecules of water and the structures can be identified by unique infrared laser spectrum signatures, according to a report in Science by Yale professor of chemistry Mark A ...

Formation of the smallest droplet of acid

Jun 19, 2009

Exactly four water molecules and one hydrogen chloride molecule are necessary to form the smallest droplet of acid. This was the result of work by the groups of Prof. Dr. Martina Havenith (physical chemistry) ...

Hydrogen ions caught in the act of wandering

Oct 05, 2005

Erik T.J. Nibbering of the Max Born Institute for Nonlinear Optics and Short Pulse Spectroscopy (MBI) and colleagues report for the first time experimental evidence of the motions of hydrogen ions (protons, H+) from acids ...

Keeping an eye on the surroundings

Aug 13, 2008

Water is no passive spectator of biological processes; it is an active participant. Protein folding is thus a self-organized process in which the actions of the solvent play a key role. So far, the emphasis ...

Recommended for you

A new approach to creating organic zeolites

Jul 24, 2014

Yushan Yan, Distinguished Professor of Engineering at the University of Delaware, is known worldwide for using nanomaterials to solve problems in energy engineering, environmental sustainability and electronics.

A tree may have the answers to renewable energy

Jul 23, 2014

Through an energy conversion process that mimics that of a tree, a University of Wisconsin-Madison materials scientist is making strides in renewable energy technologies for producing hydrogen.

User comments : 0