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Search results for fluorescence microscopy

Jun 2, 2005

New Law for Resolution Allows Unprecedented Sharpness in Fluorescence Microscopy

Researchers from the Max Planck Institute for biophysical Chemistry in Göttingen establish a new law allowing unlimited optical resolution in fluorescence microscopy Max Planck researchers have succeeded in overcoming ...

Optics & Photonics Aug 18, 2017

Super-photostable fluorescent labeling agent for super-resolution microscopy

Chemists at ITbM, Nagoya University have developed a super-photostable fluorescent dye called PhoxBright 430 (PB430) to visualize cellular ultra-structure by super-resolution microscopy. The exceptional photostability of ...

Optics & Photonics Nov 2, 2020

Single-shot 3-D wide-field fluorescence imaging with a computational miniature mesoscope

The online feature cover photograph on Science Advances this week displays fluorescence imaging with a computational miniature mesoscope (CM2). The technique of fluorescence imaging is an essential tool for biologists and ...

Optics & Photonics Feb 18, 2020

Light-sheet fluorescence imaging becomes more parallelized

An arsenal of advanced microscopy tools is now available to provide high-quality visualization of cells and organisms in 3-D and has thus substantiated our understanding the complex biological systems and functions.

Optics & Photonics Oct 13, 2023

Deep self-learning enables volumetric microscopy with 3D isotropic resolution

Volumetric fluorescence microscopy is an indispensable tool for comprehensive studies of cells and organs. Since the specimens are inherently three-dimensional (3D), the optimal imaging system should possess high spatial ...

General Physics Oct 15, 2020

Miniscope3D—A single-shot miniature three-dimensional fluorescence microscope

A miniature fluorescence microscope that weighs less while offering high resolution compared to existing devices will have a range of applications in systems biology. Existing miniature fluorescence microscopes are a standard ...

Materials Science Sep 14, 2017

Red fluorescence in two steps

Scientists have identified the mechanism that allows fluorescent proteins to switch colour in two phases. They are thereby laying the groundwork for new applications in microscopy and functional analyses in biological research.

Optics & Photonics May 12, 2021

Excitation spectral microscopy integrates multi-target imaging and quantitative biosensing

The multiplexing capability of fluorescence microscopy is severely limited by the broad fluorescence spectral width. Spectral imaging offers potential solutions, yet typical approaches to disperse the local emission spectra ...

Optics & Photonics Apr 21, 2021

Vibrational microscopy goes super resolution

True super-resolution imaging beyond the diffraction limit remains a major challenge for far-field Raman microscopy especially in biological applications. Harnessing Stimulated Raman Excited Fluorescence (SREF) as an ultrasensitive ...

Optics & Photonics Jan 28, 2019

Manipulating cell networks with light – new frontiers in optical microscopy

A new optical microscope system called stimulation and imaging-based functional optical microscopy (SIFOM) can stimulate multiple cells simultaneously via a holographic method and monitor cell activity after the stimulation ...

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