Scientists develop novel DNA logic circuits

Scientists develop novel DNA logic circuits
Fig. 1. Bipedal DNA walking based electrochemical detection of miRNA. Credit: SIBET

Based on the complementary base pairing rules, a variety of complex secondary structures can be designed and assembled to develop DNA molecular devices with specific functions which play an important role in diverse research fields.

DNA take biomolecules (e.g., DNA) or other external information as input and characterization results induced by the changes of DNA structures as output. After Boolean operations, the mutual identification and correlation of various inputs can be determined. In addition, multiple cascaded , or logic circuits, can be constructed by taking the output of the former logic gate as the input of the latter one. With the various combination and different output modes, logic circuits enjoy broad biomedical application prospects.

Recently, MIAO Peng's group from the Suzhou Institute of Biomedical Engineering and Technology (SIBET) of the Chinese Academy of Sciences has developed a novel bipedal DNA walking nanomachine, the researchers constructed a series of DNA logic circuits based on cascade strand displacement amplification. The tools can be used to explore relationships between biological molecules in complex samples.

In this research, track DNA probes with the stem-loop were modified on the electrode interface. In the upstream homogeneous system, target-triggered strand displacement amplification was introduced to generate a large number of single-stranded sequences.

Scientists develop novel DNA logic circuits
Fig. 2. Two-input logic gate construction. Credit: SIBET

The DNA three-way junction structure was then assembled as a bipedal walker. In the presence of the stem-loop structured driver strand, the bipedal walker moved around the electrode interface to enrich electrochemical signal molecules.

Furthermore, by employing incomplete three-way junction and double-stranded structures, logic gates AND and OR were constructed by cascading strand displacement amplification. Next, NAND, NOR, XOR, and XNOR gates were also developed. The two-input logic exhibited excellent performance in logic operations.

Scientists develop novel DNA logic circuits
Fig. 3. Three-input logic gate construction. Credit: SIBET

Then, the three-input AND and OR gates were fabricated using the designed four-way junction and double-stranded structures. The developed in this study can not only be applied in ultra-sensitive biomedical sensing, but also provide new ideas for researches in biomolecule information control, communication, and biological computing.

More information: Peng Miao et al, Cascade Strand Displacement and Bipedal Walking Based DNA Logic System for miRNA Diagnostics, ACS Central Science (2021). DOI: 10.1021/acscentsci.1c00277

Journal information: ACS Central Science

Citation: Scientists develop novel DNA logic circuits (2021, July 12) retrieved 25 May 2024 from
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