Nobel insight into interaction between discharge plasma and cells via TRP channel

Nobel insight into interaction between discharge plasma and cells via TRP channel
Short-lived reactive species generated by the plasma enhance the calcium influx through the TRP channels. Credit: Toshiro Kaneko

Researchers at Tohoku University have discovered that the calcium ion influx into cells, which can control the various cell activities, can be enhanced by the short-lived reactive species generated in the atmospheric pressure plasma.

This breakthrough is expected to contribute significantly to plasma medicine research - specifically, the elucidation of the mechanisms of for gene transfection, cancer therapy, blood coagulation and wound healing.

The research team has been conducting studies on low-damage and highly-efficient gene transfection - which is an indispensable tool for the generation of iPS cells and gene therapy - using the atmospheric-pressure low-temperature plasma. While it is known that gene transfection is caused by plasma irradiation, the mechanism of the plasma gene transfection has still not been clarified.

The team found that the short-lived reactive species generated in the plasma enhances through the transient receptor potential (TRP) channels that obviously contributes effectuating plasma actions including gene transfection.

The calcium influx has been found to be a trigger for the various cell activities. For example, the feeling of pain, heat, coldness in the neuron cell; the sensing of sweetness in the taste cell; and cellular response such as cell proliferation, cell differentiation, endocytosis and apoptosis via the signal transduction or cell signalling.

"It's a breakthrough to have found a way to control the calcium influx using irradiation," says Toshiro Kaneko at Tohoku University's Graduate School of Engineering. "We hope the results of our research will not only contribute to the development of low-damage and highly-efficient gene transfection, but also to improving cancer therapies, and wound healing."

Details of this study were published online on May 13 in the Scientific Reports.

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More information: Shota Sasaki et al, Calcium influx through TRP channels induced by short-lived reactive species in plasma-irradiated solution, Scientific Reports (2016). DOI: 10.1038/srep25728
Journal information: Scientific Reports

Provided by Tohoku University
Citation: Nobel insight into interaction between discharge plasma and cells via TRP channel (2016, May 13) retrieved 8 August 2020 from
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