Related topics: nanometers ยท light

Hard as a rock? Maybe not, say bacteria that help form soil

Research published this week by University of Wisconsin-Madison scientists shows how bacteria can degrade solid bedrock, jump-starting a long process of alteration that creates the mineral portion of soil.

Researchers measure wake of supersonic projectiles

Imaging technology has vastly improved over the past 30 years. It's been about that long since the flow coming off of the base of projectiles, such as ballistic missiles, has been measured. Researchers in the Department of ...

Scientists tame Josephson vortices

MIPT physicists have learned how to locally control Josephson vortices. The discovery can be used for quantum electronics superconducting devices and future quantum processors. The work has been published in the prestigious ...

Cracking the mystery of nature's toughest material

Nacre, the rainbow-sheened material that lines the insides of mussel and other mollusk shells, is known as nature's toughest material. Now, a team of researchers led by the University of Michigan has revealed precisely how ...

page 1 from 15

Thin layer chromatography

Thin layer chromatography(TLC) is a chromatography technique used to separate mixtures. Thin layer chromatography is performed on a sheet of glass, plastic, or aluminum foil, which is coated with a thin layer of adsorbent material, usually silica gel, aluminium oxide, or cellulose. This layer of adsorbent is known as the stationary phase.

After the sample has been applied on the plate, a solvent or solvent mixture (known as the mobile phase) is drawn up the plate via capillary action. Because different analytes ascend the TLC plate at different rates, separation is achieved..

Thin layer chromatography finds many applications, including:

A number of enhancements can be made to the original method to automate some steps, to increase the resolution achieved with TLC and to allow more accurate quantitation. This method is referred to as HPTLC, or "high performance TLC".

This text uses material from Wikipedia, licensed under CC BY-SA