DNA sequencing is a set of laboratory methods for determining the precise linear order of nucleotides (A, C, G, T) in a DNA molecule. Core methodological classes include Sanger sequencing, which uses chain-terminating dideoxynucleotides and capillary electrophoresis for high-fidelity, low-throughput analysis, and next-generation sequencing (NGS) platforms, which employ massively parallel sequencing-by-synthesis, sequencing-by-ligation, or nanopore-based readout for high-throughput, genome-scale applications. These methods entail library preparation, clonal or single-molecule amplification (except in true single-molecule approaches), signal detection, and computational base-calling, followed by quality control and downstream bioinformatic analysis.
From the mighty blue whale to the humble baker's yeast, scientists have barely begun to understand the vast genetic diversity among lifeforms. Of the 1.67 million known species of animal, plant, fungi and protists, just 1% ...
Plants & Animals
Sep 4, 2025
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Researchers at Beijing Genomics and IMDEA Nanociencia institutes have introduced a novel method that could significantly accelerate efficiency and reduce the cost of handling fluidics in DNA sequencing.
Biotechnology
Sep 3, 2025
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New genetic techniques are shedding light on a mysterious part of our family tree—ancient human relatives called the Denisovans that emerged during the Pleistocene epoch, approximately 370,000 years ago.
With climate change and more frequent extreme weather events, researchers predict that global yields of important crops like maize, rice, and soybeans could decline by 12 to 20% by the end of the century. To prepare, plant ...
Molecular & Computational biology
Aug 21, 2025
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Avoiding the formation of unwanted clusters of similar elements when dividing data into groups is of great importance for the analysis of medical data. Psychologists and computer scientists from Heinrich Heine University ...
Molecular & Computational biology
Aug 18, 2025
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A new study led by researchers at the University of Cambridge, in collaboration with international institutions, has uncovered a key mechanism in how DNA behaves as it passes through nanoscale pores—a process central to many ...
Bio & Medicine
Aug 14, 2025
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The sweet potato feeds millions worldwide, especially in sub-Saharan Africa, where its natural resilience to climate extremes makes it crucial for food security. But this humble root vegetable has guarded its genetic secrets ...
Plants & Animals
Aug 8, 2025
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As a molecular biologist, talking to the public about my work with DNA has often felt like trying to translate a foreign language. This got me thinking: could these scientific ideas be presented in an artistic way, to help ...
Biotechnology
Jul 30, 2025
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DNA holds the instructions that make us who we are. If we want to read those instructions, say, to understand our predisposition to genetic disorders, we need to carefully "unzip" DNA's iconic double-helix structure. This ...
Bio & Medicine
Jul 30, 2025
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70
University of Queensland researchers have developed an affordable, open-source DNA measurement tool that can be built using a home 3D printer.
Biotechnology
Jul 30, 2025
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