DNA sequencing is a set of laboratory methods for determining the precise linear order of nucleotides (A, C, G, T) in a DNA molecule. Core methodological classes include Sanger sequencing, which uses chain-terminating dideoxynucleotides and capillary electrophoresis for high-fidelity, low-throughput analysis, and next-generation sequencing (NGS) platforms, which employ massively parallel sequencing-by-synthesis, sequencing-by-ligation, or nanopore-based readout for high-throughput, genome-scale applications. These methods entail library preparation, clonal or single-molecule amplification (except in true single-molecule approaches), signal detection, and computational base-calling, followed by quality control and downstream bioinformatic analysis.
The arrival of agriculture coincided with a sharp rise in a gene variant that protected against the virus that causes winter vomiting, researchers from Karolinska Institutet and Linköping University report after analyzing ...
Evolution
Oct 24, 2025
0
107
Recent advances in long-read sequencing techniques have produced large amounts of high-quality rRNA marker gene data about eukaryotic organisms, but many of these taxa have remained unknown at the highest taxonomic levels: ...
Molecular & Computational biology
Oct 20, 2025
0
21
Preserving DNA in biological samples has long posed a challenge for researchers, but the process may be about to get a lot easier.
Biotechnology
Oct 15, 2025
0
28
Despite rapid advances in reading the genetic code of living organisms, scientists still face a major challenge today—knowing a gene's sequence does not automatically reveal what it does. Even in simple, well-studied bacteria ...
Cell & Microbiology
Oct 6, 2025
0
30
Recent studies led by an international consortium of researchers, including scientists from the San Diego Zoo Wildlife Alliance and the Museo de Historia Natural de la Universidad Nacional Mayor de San Marcos, unveil promising ...
Ecology
Oct 1, 2025
1
63
The algal blooms increasingly seen in Canadian lakes have been linked to both nutrient pollution from agricultural runoff and climate change. However, a new Concordia-led study using DNA sequencing of lakebed microbes reveals ...
Environment
Sep 30, 2025
1
22
A research team led by Professor Simona Giunta at the University of Rome La Sapienza has produced the first reference-quality genome assembly of an experimentally relevant human cell line, the widely used retinal pigment ...
Cell & Microbiology
Sep 15, 2025
0
34
Two forgotten bottles in a basement in Frederiksberg containing bacterial cultures from the 1890s have provided researchers at the University of Copenhagen with unique insight into Denmark's butter production history. Using ...
Cell & Microbiology
Sep 12, 2025
0
77
Researchers from the Belgian Federal Institute Sciensano, as part of the DARWIN project, have developed a proof-of-concept for next generation detection methods of genome-edited organisms.
Biotechnology
Sep 8, 2025
0
21
A group of scientists has sequenced the genome of a man who was buried in Egypt around 4,500 years ago. The study offers rare insight into the genetic ancestry of early Egyptians and reveals links to both ancient North Africa ...
Molecular & Computational biology
Sep 6, 2025
0
303
