DNA sequencing is a set of laboratory methods for determining the precise linear order of nucleotides (A, C, G, T) in a DNA molecule. Core methodological classes include Sanger sequencing, which uses chain-terminating dideoxynucleotides and capillary electrophoresis for high-fidelity, low-throughput analysis, and next-generation sequencing (NGS) platforms, which employ massively parallel sequencing-by-synthesis, sequencing-by-ligation, or nanopore-based readout for high-throughput, genome-scale applications. These methods entail library preparation, clonal or single-molecule amplification (except in true single-molecule approaches), signal detection, and computational base-calling, followed by quality control and downstream bioinformatic analysis.
Scientists have uncovered new DNA-binding proteins from some of the most extreme environments on Earth and shown that they can improve rapid medical tests for infectious diseases. The work has been published in Nucleic Acids ...
Biotechnology
Feb 10, 2026
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Cucumber is an economically important crop worldwide, ranking as the third most-produced vegetable after tomatoes and onions. Yet breeding improved varieties—plants that are more resilient, produce better-shaped fruit, or ...
Plants & Animals
Feb 10, 2026
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Scientists at the Department of Energy's Oak Ridge National Laboratory have developed software that reduces the time needed for a key task in the development of custom microbes from a week to just hours. The new tool cracks ...
Biotechnology
Feb 9, 2026
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A large-scale population metagenomic study has shed new light on the spatial heterogeneity of viral communities across the gastrointestinal tracts of ruminants, which are closely linked to human history. The team, led by ...
Ecology
Feb 6, 2026
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Bacteria in food can make you seriously ill, which is why it is so important for the facilities that produce your food to ensure proper hygiene in their production lines. A new doctoral thesis from NTNU has investigated how ...
Cell & Microbiology
Feb 5, 2026
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Ferns, defined by large genomes, high chromosome counts, and pervasive aneuploidy as well as intraspecific polyploid complexity, diverge significantly from the classical genetic theories and analytical frameworks largely ...
Plants & Animals
Feb 3, 2026
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14
Artificial intelligence allows tracing the evolution of genetic control elements in the developing mammalian cerebellum. An international research team led by biologists from Heidelberg University as well as the Vlaams Instituut ...
Evolution
Jan 29, 2026
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49
Researchers at Forschungszentrum Jülich and Heinrich Heine University Düsseldorf have developed a tool that could significantly transform genome research: Helixer identifies genes directly from DNA sequences—without laboratory ...
Molecular & Computational biology
Jan 26, 2026
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16
The development of humans and other animals unfolds gradually over time, with cells taking on specific roles and functions via a process called cell fate determination. The fate of individual cells, or in other words, what ...
Johns Hopkins University geneticists and a small army of researchers across the country, including students, are working to catalog the vast and largely unknown soil microbiome of the United States.
Ecology
Jan 6, 2026
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