DNA sequencing is a set of laboratory methods for determining the precise linear order of nucleotides (A, C, G, T) in a DNA molecule. Core methodological classes include Sanger sequencing, which uses chain-terminating dideoxynucleotides and capillary electrophoresis for high-fidelity, low-throughput analysis, and next-generation sequencing (NGS) platforms, which employ massively parallel sequencing-by-synthesis, sequencing-by-ligation, or nanopore-based readout for high-throughput, genome-scale applications. These methods entail library preparation, clonal or single-molecule amplification (except in true single-molecule approaches), signal detection, and computational base-calling, followed by quality control and downstream bioinformatic analysis.
Researchers have developed VIRE, a database that integrates approximately 1.7 million viral genomes derived from more than 100,000 metagenomes worldwide. Metagenomic data is obtained by comprehensively sequencing all DNA ...
Molecular & Computational biology
Dec 1, 2025
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A major milestone has been reached, with experts across Europe, including those at the Wellcome Sanger Institute in Cambridge, UK, sequencing 1,000 species of butterflies and moths. This includes almost all UK butterflies, ...
Evolution
Nov 30, 2025
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Cornell researchers have found that a new DNA sequencing technology can be used to study how transposons move within and bind to the genome. Transposons play critical roles in immune response, neurological function and genetic ...
Biotechnology
Nov 21, 2025
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Mitochondria generate most of the body's energy. Variations in their genetic material have long been linked to neurological disorders, metabolic syndromes, and cancers. However, most large-scale mitochondrial DNA (mtDNA) ...
Cell & Microbiology
Nov 20, 2025
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Researchers from Stockholm University have—for the first time ever—managed to successfully isolate and sequence RNA molecules from Ice Age woolly mammoths. These RNA sequences are the oldest ever recovered and come from mammoth ...
Molecular & Computational biology
Nov 14, 2025
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175
Research institutions around the world house valuable genetic information that could help unlock countless medical mysteries. However, because DNA degrades over time, it is difficult for researchers to analyze DNA samples ...
Biotechnology
Nov 13, 2025
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A research team led by the A*STAR Genome Institute of Singapore (A*STAR GIS) have developed a method to accurately and efficiently read DNA containing non-standard bases—a task once thought too complex for conventional DNA ...
Bio & Medicine
Nov 13, 2025
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91
Traditional bisulfite sequencing damages DNA, while enzyme-based alternatives are inconsistent. A novel methylation analysis method, called UMBS-seq, has been published in Nature Communications.
Biotechnology
Nov 13, 2025
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20
QUT researchers have recovered the genomes of more than 24,000 previously unknown microbial species—some from entirely new branches of life that likely evolved before plants and animals. The microbes are detailed in two studies ...
Cell & Microbiology
Nov 13, 2025
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34
Seed plants are essential as a source of food, fuel, medicine, and more. Now, a multidisciplinary team of researchers has combined deep botanical knowledge with powerful genomic technology to decode and mine the DNA of non-flowering ...
Molecular & Computational biology
Nov 13, 2025
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