DNA sequencing is a set of laboratory methods for determining the precise linear order of nucleotides (A, C, G, T) in a DNA molecule. Core methodological classes include Sanger sequencing, which uses chain-terminating dideoxynucleotides and capillary electrophoresis for high-fidelity, low-throughput analysis, and next-generation sequencing (NGS) platforms, which employ massively parallel sequencing-by-synthesis, sequencing-by-ligation, or nanopore-based readout for high-throughput, genome-scale applications. These methods entail library preparation, clonal or single-molecule amplification (except in true single-molecule approaches), signal detection, and computational base-calling, followed by quality control and downstream bioinformatic analysis.
The development of humans and other animals unfolds gradually over time, with cells taking on specific roles and functions via a process called cell fate determination. The fate of individual cells, or in other words, what ...
Johns Hopkins University geneticists and a small army of researchers across the country, including students, are working to catalog the vast and largely unknown soil microbiome of the United States.
Ecology
Jan 6, 2026
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Scientists and physicians can better assess precision genome editing technology using a new method made public today by St. Jude Children's Research Hospital. Significant amounts of time and resources spent improving CRISPR ...
Biotechnology
Jan 3, 2026
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115
Agriculture, from the outset, has been made possible by humans tweaking the genes of plants to make them grow faster, produce more of what we want, and survive drought, pests, and infection. For millennia, we did it with ...
Biotechnology
Dec 18, 2025
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24
DNA captured on air filters and stored since the 1960s acts as an ecological time capsule, according to a recent publication in Nature Communications. The findings show that tiny fragments of genetic material can paint a ...
Ecology
Dec 18, 2025
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34
Imagine a container of tomatoes arriving at the container terminal in Aarhus. The papers state that the tomatoes are from Spain, but in reality, we have no way of knowing if that is true.
Biotechnology
Dec 17, 2025
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20
The microscopic organisms that fill our bodies, soils, oceans and atmosphere play essential roles in human health and the planet's ecosystems. Yet even with modern DNA sequencing, figuring out what these microbes are and ...
Cell & Microbiology
Dec 12, 2025
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66
More than a decade after the first Neanderthal genome was sequenced, scientists are still working to understand how human-specific DNA changes shaped human evolution.
Evolution
Dec 10, 2025
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DNA sequencing technology makes it possible to explore the genome to learn how humans adapted to live in a wide range of environments. Research has shown, for instance, that Tibetans living at high altitude in the Himalayas ...
Evolution
Dec 3, 2025
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132
The last two decades have seen a revolution in scientists' ability to reconstruct the past. This has been made possible through technological advances in the way DNA is extracted from ancient bones and analyzed.
Molecular & Computational biology
Dec 2, 2025
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