DNA sequencing is a set of laboratory methods for determining the precise linear order of nucleotides (A, C, G, T) in a DNA molecule. Core methodological classes include Sanger sequencing, which uses chain-terminating dideoxynucleotides and capillary electrophoresis for high-fidelity, low-throughput analysis, and next-generation sequencing (NGS) platforms, which employ massively parallel sequencing-by-synthesis, sequencing-by-ligation, or nanopore-based readout for high-throughput, genome-scale applications. These methods entail library preparation, clonal or single-molecule amplification (except in true single-molecule approaches), signal detection, and computational base-calling, followed by quality control and downstream bioinformatic analysis.
Antibiotics are one of the greatest triumphs in the history of medical science—but these lifesaving tools have a dark side. Their persistent use can produce "superbugs"—drug-resistant microbes that pose a danger to humans, ...
Biotechnology
Jun 27, 2025
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Scientists at UCLA and the University of Toronto have developed an advanced computational tool, called moPepGen, that helps identify previously invisible genetic mutations in proteins, unlocking new possibilities in cancer ...
Biotechnology
Jun 16, 2025
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A pioneering partnership between researchers from The Grainger College of Engineering at the University of Illinois Urbana-Champaign has produced a novel nanopore sensing platform for single-biomolecule detection. Their findings, ...
Bio & Medicine
Jun 12, 2025
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Over the last 10 years, breakthroughs in understanding the genetic instructions passed from parent to offspring have put researchers closer than ever before to efficiently decoding DNA with 100% accuracy. However, this analysis ...
Plants & Animals
Jun 12, 2025
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Scientists at St. Jude Children's Research Hospital, the National Center for Genomic Analysis and the University of Adelaide have created a single-cell RNA analysis method that is 47 times cheaper and more scalable than other ...
Cell & Microbiology
Jun 11, 2025
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105
Bigelow Laboratory scientists have advanced an exciting method for linking the activity of individual microbes to their unique genetic code, providing the first application of the approach to sediments. Their findings were ...
Environment
Mar 14, 2025
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A team of researchers from NUS has developed a novel method to enhance the precision of cancer treatment using gold nanoparticles tagged with DNA barcodes.
Bio & Medicine
Jan 24, 2025
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Different types of cancer have unique molecular "fingerprints" which are detectable in early stages of the disease and can be picked up with near-perfect accuracy by small, portable scanners in just a few hours, according ...
Bio & Medicine
Dec 10, 2024
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Transmembrane β-barrel pores (TMBs) are extensively used for single-molecule DNA and RNA sequencing. They enable the miniaturization of a wide array of sensing and sequencing applications into portable USB-size devices and ...
Bio & Medicine
Jul 18, 2024
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121
UMass Amherst researchers have pushed forward the boundaries of biomedical engineering one hundredfold with a new method for DNA detection with unprecedented sensitivity.
Bio & Medicine
Oct 3, 2023
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