DNA sequencing is a set of laboratory methods for determining the precise linear order of nucleotides (A, C, G, T) in a DNA molecule. Core methodological classes include Sanger sequencing, which uses chain-terminating dideoxynucleotides and capillary electrophoresis for high-fidelity, low-throughput analysis, and next-generation sequencing (NGS) platforms, which employ massively parallel sequencing-by-synthesis, sequencing-by-ligation, or nanopore-based readout for high-throughput, genome-scale applications. These methods entail library preparation, clonal or single-molecule amplification (except in true single-molecule approaches), signal detection, and computational base-calling, followed by quality control and downstream bioinformatic analysis.
Today (May 22) is United Nations International Day for Biological Diversity, drawing attention to a critical resource for developing crops that are resilient or resistant to extreme weather and other threats to their health, ...
Plants & Animals
May 22, 2026
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Beekeepers and their honeybees can be invaluable participants in environmental surveys, according to a study published in the open-access journal PLOS One by Jennifer Shelton of the UK Center for Ecology & Hydrology and colleagues.
Plants & Animals
May 20, 2026
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Scientists have long known that the DNA code in genes is not the only way to pass genetic traits from parents to offspring. "Epigenetic" marks—chemical modifications to DNA that don't change the DNA code itself—can also be ...
Molecular & Computational biology
May 20, 2026
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Parasitic nematodes (commonly known as roundworms) are a large, diverse and poorly studied group of disease-causing organisms that severely impact the health of humans and animals. They infect almost one-quarter of the global ...
Cell & Microbiology
May 19, 2026
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DNA is everywhere in the world's oceans—not only packaged inside cells from skin, scales, mucus, feces, and blood, but also floating freely. Sequencing such "environmental DNA" (eDNA) from open water has long been used as ...
Ecology
May 19, 2026
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Researchers at the Max Planck Institute for Biology Tübingen have shown that giant viruses long thought to exist only as fleeting, free-living particles that can embed themselves permanently in the genome of a multicellular ...
Evolution
May 18, 2026
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Gathering dust from buildings may hold promise as a more efficient way to track viral outbreaks in indoor settings, according to a new study published in Building and Environment by researchers from the Ohio State University. ...
Cell & Microbiology
May 18, 2026
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Guide dogs help thousands of people with visual disabilities navigate daily life. While guide dogs provide tremendous benefits, the current training program faces serious inefficiencies, since a large percentage never actually ...
Molecular & Computational biology
May 15, 2026
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The genomics of peppermint are not as fresh as their flavor but scientists from the University of California, Davis, have found a way to breathe new genetic variation into the species. The findings, published in the Proceedings ...
Biotechnology
May 14, 2026
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A new genetic test has revealed that most of the free-roaming canines in Australia, often labeled "wild dogs," carry a significant amount of dingo ancestry. A team of Adelaide University researchers from the Australian Center ...
Ecology
May 14, 2026
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