DNA sequencing is a set of laboratory methods for determining the precise linear order of nucleotides (A, C, G, T) in a DNA molecule. Core methodological classes include Sanger sequencing, which uses chain-terminating dideoxynucleotides and capillary electrophoresis for high-fidelity, low-throughput analysis, and next-generation sequencing (NGS) platforms, which employ massively parallel sequencing-by-synthesis, sequencing-by-ligation, or nanopore-based readout for high-throughput, genome-scale applications. These methods entail library preparation, clonal or single-molecule amplification (except in true single-molecule approaches), signal detection, and computational base-calling, followed by quality control and downstream bioinformatic analysis.
A research team led by Zhen-Xing Endowed Professor Jian Yang at the School of Life Sciences, Westlake University, has developed a pangenome-informed genome assembly (PIGA) method. By combining a cost-effective hybrid sequencing ...
Molecular & Computational biology
Apr 3, 2026
0
55
August Weismann's germ plasm theory of the late 19th century posited that only germ cells, e.g., sperm and egg cells in animals or pollen and ovule cells in plants, transmit genetic information to the next generation, and ...
Plants & Animals
Apr 2, 2026
0
30
New research has discovered that the molecular machines responsible for copying our DNA have a surprising hidden talent—an ability to create entirely new and highly sophisticated DNA sequences from scratch. The study, led ...
Biotechnology
Apr 1, 2026
0
99
Scientists have uncovered an unexpected microbial relationship that could help explain differences in the severity of a major disease affecting common beans. The discovery sheds light on how the pathogen evolves and may point ...
Ecology
Mar 30, 2026
0
135
Desperate to help his sick dog, one Australian man went down the ultimate ChatGPT research hole, using artificial intelligence to design a personalized experimental treatment and finding top scientists to administer it.
Veterinary medicine
Mar 30, 2026
0
22
Neanderthals split into distinct regional groups that developed genetic differences far sooner than modern human populations typically did, according to a study published in Proceedings of the National Academy of Sciences. ...
The bond between humans and dogs is one of nature's most enduring partnerships, but exactly when it began has long been a mystery. Now, a new study has turned back the clock. The study, titled "Dogs were widely distributed ...
Evolution
Mar 28, 2026
0
44
Understanding gene expression within the body has been a boon for 21st century biology and therapeutics, but most discoveries that use these technologies only focus on one organ or one small area of tissue. At the University ...
Cell & Microbiology
Mar 27, 2026
0
29
Anopheles darlingi mosquitoes—a major vector of malaria in South America—are evolving in response to insecticides, which may make them harder to kill and malaria more difficult to control, according to a new study led by ...
Plants & Animals
Mar 26, 2026
0
8
Scientists at McMaster University have uncovered new evidence that the fungus causing white-nose syndrome, a deadly disease affecting bats, may be more widespread in Western Canada than existing testing methods reveal. In ...
Plants & Animals
Mar 25, 2026
0
10
