Blending bacterial genomes for megacloning

Oct 18, 2005

Scientists in Tokyo report developing a "megacloning" method of transferring entire genomes from one bacterial species into another.

DNA bacterial cloning is routinely used to isolate genes and understand gene function, but most bacteria cannot handle a large number of genes at one time.

In an attempt to increase the genomic size for DNA cloning, Mitsuhiro Itaya and colleagues from the Institute of Life Sciences made use of a DNA cloning vector derived from the 4.2-Mb sized genome of the Bacillus subtilis bacteria, and for the target DNA used the 3.5-Mb genome of the non-pathogenic, photosynthetic bacterium Synechocystis.

The researchers divided the Synechocystis genome into four regions of approximately equal size and guided that target DNA into the cloning vector by genetic recombination at "landing pad sequences."

Through progressive assembly and editing of DNA regions covering the entire Synechocystis genome sequence, the resultant bacteria contained a composite genome size of 7.7 Mb.

The scientists said their megacloning method extends size limits of stable DNA cloning and might lead to construction of beneficial microbes for bioindustrial use.

Their research appears in the online early edition of the Proceedings of the National Academy of Sciences.

Copyright 2005 by United Press International

Explore further: Awarded a Pell Grant? Better double-check

add to favorites email to friend print save as pdf

Related Stories

Rainbow trout genome sequenced

Apr 22, 2014

Using fish bred at Washington State University, an international team of researchers has mapped the genetic profile of the rainbow trout, a versatile salmonid whose relatively recent genetic history opens ...

Androgenetic species of clam utilizes rare gene capture

May 24, 2011

(PhysOrg.com) -- In a new study published in the Proceedings of the National Academy of Sciences, biologist David Hillis from the University of Texas shows how the freshwater Corbicula clam utilizes rare g ...

Recommended for you

Awarded a Pell Grant? Better double-check

3 hours ago

(AP)—Potentially tens of thousands of students awarded a Pell Grant or other need-based federal aid for the coming school year could find it taken away because of a mistake in filling out the form.

Ultra high definition TVs boost LG Display profit

11 hours ago

(AP)—LG Display Co. said profit for the April-June quarter more than doubled as a stronger won reduced the value of its foreign debt and the World Cup boosted demand for ultra-high-definition TVs.

Drugmaker GSK slashes annual profits forecast

11 hours ago

British drugmaker GlaxoSmithKline on Wednesday slashed its 2014 profits forecast as second-quarter earnings sank on the back of weak US trade, adverse currency moves and a Chinese bribery probe.

Perthites wanted for study on the Aussie lingo

11 hours ago

We all know that Australians speak English differently from the way it's spoken in the UK or the US, and many of us are aware that Perth people have a slightly different version of the language from, say, Melbournians - but ...

User comments : 0