Blending bacterial genomes for megacloning

Oct 18, 2005

Scientists in Tokyo report developing a "megacloning" method of transferring entire genomes from one bacterial species into another.

DNA bacterial cloning is routinely used to isolate genes and understand gene function, but most bacteria cannot handle a large number of genes at one time.

In an attempt to increase the genomic size for DNA cloning, Mitsuhiro Itaya and colleagues from the Institute of Life Sciences made use of a DNA cloning vector derived from the 4.2-Mb sized genome of the Bacillus subtilis bacteria, and for the target DNA used the 3.5-Mb genome of the non-pathogenic, photosynthetic bacterium Synechocystis.

The researchers divided the Synechocystis genome into four regions of approximately equal size and guided that target DNA into the cloning vector by genetic recombination at "landing pad sequences."

Through progressive assembly and editing of DNA regions covering the entire Synechocystis genome sequence, the resultant bacteria contained a composite genome size of 7.7 Mb.

The scientists said their megacloning method extends size limits of stable DNA cloning and might lead to construction of beneficial microbes for bioindustrial use.

Their research appears in the online early edition of the Proceedings of the National Academy of Sciences.

Copyright 2005 by United Press International

Explore further: Sundance doc examines real-life Close Encounter

add to favorites email to friend print save as pdf

Related Stories

Scientists resurrect 700-year-old viruses

Oct 28, 2014

(Phys.org) —Eric Delwart of the Blood Systems Research Institute in San Francisco and colleagues have found two 700-year-old viral sequences in frozen caribou dung in an arctic ice patch. The researchers ...

Androgenetic species of clam utilizes rare gene capture

May 24, 2011

(PhysOrg.com) -- In a new study published in the Proceedings of the National Academy of Sciences, biologist David Hillis from the University of Texas shows how the freshwater Corbicula clam utilizes rare g ...

Recommended for you

Sundance doc examines real-life Close Encounter

7 hours ago

Earth authorities are completely unprepared for the arrival of alien visitors and worried humans should ready themselves by watching a groundbreaking documentary, the film's director boasts.

Is this the year you join the one percent?

8 hours ago

Here's some good news for the New Year: According to new research by Washington University in St. Louis and Cornell University, there's a 1 in 9 chance that a typical American will hit the jackpot and join ...

User comments : 0

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.