New DNA Tool Probes Rice Genome

October 21, 2008,

(PhysOrg.com) -- A new tool for investigating the rice genome has been developed by researchers at UC Davis led by Pamela Ronald, professor of plant pathology. The inexpensive, publicly-available rice DNA microarray covers nearly all the 45,000 genes in the rice genome. Details are published this week in the journal PLoS (Public Library of Science) One.

In higher organisms, like humans or rice plants, each cell type express different genes at different times. Scientists have developed high-throughput methods to examine these gene expression profiles using "DNA microarrays," thousands of fragments of DNA fixed to a glass slide. DNA microarrays can be used to figure out which genes are important for responding to a stimulus or tolerating stresses.

Ronald and her colleagues used the new rice array to investigate gene expression changes when plants are grown in the light versus the dark. They then combined this gene expression data with biochemical pathway data to correctly predict a number of candidate gene products involved in carrying out photorespiration.

The methods and array developed in this paper will aid researchers in identifying the function of the 45,000 rice genes, only a few of which have so far been characterized, Ronald said. The group also has developed a Web-based program that allows the user to compare gene expression profiles across multiple rice microarray platforms, which will further accelerate this research.

Provided by UC Davis

Explore further: To untangle the effects of nanoparticles on microbes, look at the genes

Related Stories

Team reports progress in pursuit of sickle cell cure

February 16, 2018

Scientists have successfully used gene editing to repair 20 to 40 percent of stem and progenitor cells taken from the peripheral blood of patients with sickle cell disease, according to Rice University bioengineer Gang Bao.

Recommended for you

New interaction mechanism of proteins discovered

February 22, 2018

UZH researchers have discovered a previously unknown way in which proteins interact with one another and cells organize themselves. This new mechanism involves two fully unstructured proteins forming an ultra-high-affinity ...

0 comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.