Single molecule techniques comprise a set of experimental methods that detect, manipulate, and analyze individual biomolecules or molecular complexes, circumventing ensemble averaging and revealing heterogeneity in structure, dynamics, and function. Core modalities include single-molecule fluorescence (e.g., smFRET, TIRF microscopy), optical and magnetic tweezers, atomic force microscopy, and nanopore-based sensing. These techniques enable direct measurement of forces, conformational changes, binding/unbinding events, and reaction pathways with nanometer spatial and millisecond (or better) temporal resolution. They are widely applied to study nucleic acid–protein interactions, molecular motors, enzyme kinetics, folding energy landscapes, and mechanotransduction at the level of individual molecules.
Proteins are responsible for most functions in the human body. However, their analysis, which is essential for understanding diseases, developing drugs and discovering new biomarkers, remains highly complex. Using a technology ...
Bio & Medicine
Jun 29, 2026
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By teaching an AI to use optical tweezers, researchers from the University of Gothenburg and Chalmers University of Technology have sped up the analysis of life's smallest components. The AI platform captures particles, takes ...
Bio & Medicine
Jun 18, 2026
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Using an innovative combination of biochemical experiments and ultra-high-resolution microscopy, a research team at Kiel University has solved the long-standing mystery of how the bacterium B. subtilis regulates its cell ...
Cell & Microbiology
Jun 16, 2026
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A new single-protein analysis technique gives researchers an unprecedented ability to study proteins called scramblases, which have critical roles in biology. The development of the new technique, in a study led by investigators ...
Cell & Microbiology
Jun 15, 2026
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Thanks to effective therapies, more and more people are now able to live with or after cancer in the long term. Consequently, the number of patients affected by the long-term effects of their treatment is also increasing. ...
Molecular & Computational biology
Jun 8, 2026
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DNA is the blueprint of the human body. However, tens of thousands of DNA lesions occur in our bodies every day. In particular, if "apurinic/apyrimidinic sites" (AP sites, damaged sites where one letter of DNA information ...
Cell & Microbiology
Jun 4, 2026
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In a study published in ACS Nano, researchers from National Taiwan University report a new expansion microscopy strategy termed high-fold homogeneous expansion microscopy (hiHomoExM), capable of achieving approximately 8–9× ...
Bio & Medicine
May 26, 2026
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Precision medicine aims to transport therapeutic agents, such as molecules, proteins or RNA, to the exact place where they need to act within the body. One of the most promising strategies is the use of nanocarriers: nanoparticles ...
Bio & Medicine
May 21, 2026
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Researchers from A*STAR Genome Institute of Singapore (A*STAR GIS) have developed a new method to study individual RNA molecules and reveal how their structures influence gene regulation, a fundamental process that affects ...
Biotechnology
May 15, 2026
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Using a powerful single-molecule imaging method they developed, a Broad Institute research team has unveiled a dynamic view of how some cancer-related proteins interact in living cells. The technique relies on highly stable ...
Bio & Medicine
May 4, 2026
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