Polymerase chain reaction (PCR) is a nucleic acid amplification method that enzymatically generates large quantities of a specific DNA fragment from minimal starting material through iterative thermal cycling. The technique relies on sequence-specific oligonucleotide primers, a thermostable DNA polymerase, deoxynucleoside triphosphates, and defined cycling parameters comprising denaturation, primer annealing, and extension steps. PCR enables exponential amplification of target sequences with high sensitivity and specificity, supporting downstream applications such as cloning, sequencing, mutational analysis, quantitative nucleic acid measurements, and detection of low-abundance or degraded genetic material in research, diagnostics, forensics, and environmental monitoring.
According to experts, bringing an end to the pandemic will require rapid screening of people attending large gatherings, such as conferences and weddings. Even those who are asymptomatic can still transmit COVID-19 to others, ...
Bio & Medicine
Feb 2, 2022
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