Environmental DNA (eDNA) refers to genetic material obtained directly from environmental samples such as water, soil, air, or sediments, without isolating target organisms. It consists of extracellular DNA and cellular fragments shed through excretion, secretion, reproduction, or decomposition. eDNA-based methods typically involve sample collection, DNA extraction, amplification of taxonomically informative genetic markers (e.g., mitochondrial or ribosomal loci), and high-throughput sequencing or qPCR for detection and quantification. This topic encompasses methodological optimization, degradation kinetics, transport dynamics, contamination control, and bioinformatic pipelines, and is central to non-invasive biodiversity assessment, community composition profiling, invasive species surveillance, and ecological monitoring across aquatic and terrestrial ecosystems.
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