Ecotoxicology is the scientific discipline that studies the fate and effects of chemical, physical, and biological stressors on organisms, populations, communities, and ecosystems in the environment. It integrates toxicology, ecology, environmental chemistry, and risk assessment to quantify exposure, dose–response relationships, and adverse outcome pathways under realistic environmental conditions. Ecotoxicology addresses contaminant transport, transformation, bioavailability, bioaccumulation, and biomagnification across trophic levels, and evaluates sublethal endpoints such as endocrine disruption, behavioral changes, and reproductive impairment. The field underpins environmental quality criteria, ecological risk assessment, and regulatory decision-making for pollutants including pesticides, metals, pharmaceuticals, and emerging contaminants.