DNA sequencing is a set of laboratory methods for determining the precise linear order of nucleotides (A, C, G, T) in a DNA molecule. Core methodological classes include Sanger sequencing, which uses chain-terminating dideoxynucleotides and capillary electrophoresis for high-fidelity, low-throughput analysis, and next-generation sequencing (NGS) platforms, which employ massively parallel sequencing-by-synthesis, sequencing-by-ligation, or nanopore-based readout for high-throughput, genome-scale applications. These methods entail library preparation, clonal or single-molecule amplification (except in true single-molecule approaches), signal detection, and computational base-calling, followed by quality control and downstream bioinformatic analysis.
DNA holds the instructions that make us who we are. If we want to read those instructions, say, to understand our predisposition to genetic disorders, we need to carefully "unzip" DNA's iconic double-helix structure. This ...
Bio & Medicine
Jul 30, 2025
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University of Queensland researchers have developed an affordable, open-source DNA measurement tool that can be built using a home 3D printer.
Biotechnology
Jul 30, 2025
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An international team of scientists has decoded some of the most stubborn, overlooked regions of the human genome using complete sequences from 65 individuals across diverse ancestries.
Biotechnology
Jul 23, 2025
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A world-first tool is tracking Australia's progress in sequencing the full genomes—or genetic blueprints—of the country's 250,000-plus known and catalogued species.
Molecular & Computational biology
Jul 23, 2025
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Since the Human Genome Project first produced the genetic instructions for a human being by sequencing DNA 22 years ago, scientists have been focused on roughly 2% of the genome-producing proteins.
Molecular & Computational biology
Jul 17, 2025
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24
A team that includes Rutgers University-New Brunswick scientists has unlocked some of the secrets of corn DNA, revealing how specific sections of genetic material control vital traits such as plant architecture and pest resistance.
Molecular & Computational biology
Jul 15, 2025
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Scientists have sequenced the complete genome of a roundworm used extensively in biological research, opening a new pathway for synthetic biologists to build and test genetic changes in a multicellular animal species. The ...
Molecular & Computational biology
Jul 15, 2025
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20
Creating genomes from DNA is hugely important for managing threatened species—understanding their past and how they have adapted to their environment helps conservationists protect them.
Plants & Animals
Jul 8, 2025
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29
Imagine that you want to know the plot of a movie, but you only have access to either the visuals or the sound. With visuals alone, you'll miss all the dialog. With sound alone, you will miss the action. Understanding our ...
Biotechnology
Jul 1, 2025
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28
Using whole genome sequencing and cutting‐edge analyses, researchers at Stockholm University have uncovered the surprising evolutionary history of the Norwegian lemming (Lemmus lemmus), revealing it to be one of the most ...
Plants & Animals
Jun 30, 2025
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