Biodegradation is the biologically mediated transformation and mineralization of organic or inorganic substances by microorganisms, such as bacteria, fungi, and archaea, often via extracellular and intracellular enzymatic pathways. It proceeds through sequential steps including depolymerization, primary degradation to smaller intermediates, and ultimate mineralization to CO₂, CH₄, H₂O, inorganic salts, and biomass under aerobic or anaerobic conditions. In environmental and engineering contexts, biodegradation governs the fate of pollutants, natural organic matter, and synthetic materials, and is a central process in bioremediation, wastewater treatment, and life-cycle assessment of chemicals and polymers.
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