Enzyme inhibitors block replication of SARS virus

Mar 28, 2006

The study was conducted by researchers from Scripps Research; the Genomics Research Center, Academia Sinica, Taiwan; and the National Taiwan University. It is being published today in the journal Chemistry and Biology.

Chi-Huey Wong is currently the Ernest W. Hahn Chair in Chemistry at the Skaggs Institute of Chemical Biology and directs the Scripps Research lab heading the study. He said the new finding is an important step in developing a possible drug treatment against SARS.

"We have been working on the problem of SARS since the epidemic started in 2003," Wong said. "This new class of inhibitors represents the most potent SARS virus protease inhibitors known today."

The path to today's research finding has taken several years. In 2002, Severe Acute Respiratory Syndrome (SARS) emerged in rural China and eventually spread to 32 countries, according to the World Health Organization. SARS is caused by a ring-shaped virus, known as a coronavirus. The SARS coronavirus is suspected of originating in animal populations before migrating to humans. Hardest-hit were six Asian nations. By the time the epidemic had been controlled in 2003, the disease infected more than 8,000 people, causing 800 deaths. There is no current effective treatment or vaccine.

Researchers have known since 2003 that a site on the virus is responsible for mediating proteases that allow the virus to replicate. Since then researchers have been testing protease inhibitors to lock up this site, known as SARS 3CLpro, and effectively stop the virus from infecting additional cells in the body.

In 2004, Wong's lab discovered that Lopinavir, a protease inhibitor of HIV also known as TL3, also served as weak inhibitor of the SARS 3CLpro site (PNAS, 101, 10012-10017). Since then, members of Wong's group further studied Lopinavir and are preparing it for clinical trials against SARS.

Researchers in Wong's lab at Scripps Research and in Taiwan have been looking at other Liponavir-related compounds for similar blocking effects. During these experiments, they found that a group of catalyzing agents used to help promote chemical reactions in the laboratory were actually more powerful in blocking the SARS protease than either the Lopinavir or any of the target compounds.

These organic compounds are called benzotriazole esters. The esters entered the SARS protease site, formed an intermediary compound, then inactivated the SARS enzyme. The findings were confirmed using mass spectrometry analysis of the enzyme intermediary.

"These benzotriazole esters are relatively stable and act as suicide inhibitors," Wong said. "They block the enzyme, are transformed through a co-valent bond, and are unable to get out."

Wong said the findings published today provide better insight into the mode of action of the enzyme, which may lead to development of a drug against SARS. The findings were made by using rapid drug discovery techniques developed in the Wong lab to screen large numbers of weak enzyme inhibitors, and then attaching additional compounds to look for stronger reactions.

Research Associate Chung-Yi Wu, a member of the Wong lab, is the paper's lead author. He said the finding was unexpected.

"We wanted to improve Liponavir activity," Wu said. "But we found this very surprising and serendipitous result."

Source: Scripps Research Institute

Explore further: Science magazine retracts study on voters' gay-rights views

Related Stories

Godwits are flexible... when they get the chance

3 hours ago

Black-tailed godwits are able to cope with unpredictable weather. This was revealed by a thorough analysis of the extraordinary spring of 2013 by ecologist Nathan Senner of the University of Groningen and ...

Recommended for you

Why do people waste so much time at work?

21 minutes ago

Based on research undertaken at Cass Business School, City University London, Professor Fleming explores how the act of working is no longer about survival and self-preservation, but has now morphed into ...

High finance, high anxiety

57 minutes ago

Studying events like the 2008 financial crisis in hindsight leads many to ask, "How could they have gotten it so wrong?"

Researchers provide evolutionary explanation of crime

1 hour ago

In their new book, "Evolutionary criminology: Towards a comprehensive explanation of crime," Dr Russil Durrant from the Institute of Criminology and Professor Tony Ward from the School of Psychology employ ...

Study suggests that dinosaurs were warm-blooded

2 hours ago

Dinosaurs grew as fast as your average living mammal, according to a research paper published by Stony Brook University paleontologist Michael D'Emic, PhD. The paper, to published in Science on May 29, is ...

User comments : 0

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.