Thousands of New Proteins Discovered in Spinal Fluid

Jul 16, 2010
A U.S. and Swedish team identified 2,630 proteins in the clear fluid that protects the brain and spinal cord.

(PhysOrg.com) -- Scientists and doctors have a far better understanding of the proteins in healthy spinal fluid, thanks to a U.S., Swedish team who identified 2,630 proteins in the clear fluid that protects the brain and spinal cord. This discovery nearly triples the number of proteins known to exist in spinal fluid. Another striking finding was that slightly more than half of the proteins were not found in blood.

The team was led by Richard D. Smith, Ph.D., of Pacific Northwest National Laboratory, and Steven E. Schutzer, MD, of the University of Medicine and Dentistry of New Jersey -- New Jersey Medical School.

In conducting this research, the team used integrated resources at EMSL that included a custom-built automated nanocapillary liquid chromatography system coupled on-line to one of two mass spectrometers, modified in-house with an electrodynamic ion funnel.

This research establishes a reference database that may help researchers and clinicians determine the root causes of Alzheimer's, Parkinson's, and other . In addition, this work could improve the chances of devising faster, more efficient diagnostic tests and treatments for diseases with neurological and psychiatric features.

While this work is the most comprehensive characterization of normal to date, it is only the beginning. Quickly and precisely determining the proteins in different samples opens doors to fundamentally understanding human health.

"These exciting findings are the tip of our research iceberg," said Smith, a Wiley Research Fellow at the Department of Energy's EMSL.

Explore further: Crystal structure reveals how minor variations make receptor proteins activate or inhibit natural killer cells

More information: Schutzer SE, T Liu, BH Natelson, TE Angel, AA Schepmoes, SO Purvine, KK Hixson, MS Lipton, DG Camp II, PK Coyle, RD Smith, and J Bergquist. 2010. "Establishing the Proteome of Normal Human Cerebrospinal Fluid." PLoS One 5(6):e10980. DOI: 10.1371/journal.pone.0010980

add to favorites email to friend print save as pdf

Related Stories

Spinal fluid proteins signal Lou Gehrig's disease

Jan 28, 2009

High levels of certain proteins in the spinal fluid could signal the onset of Lou Gehrig's disease, according to researchers. The discovery of these biomarkers may lead to diagnostic kits for early diagnosis, accurately measuring ...

New, sought-after standard for diagnosis within neurology

Jun 15, 2010

For the first time, researchers from Sweden and the US have succeeded in identifying the majority of the detectable proteins in the cerebrospinal fluid (CSF) of healthy human beings. The advance is expected to provide a valuable ...

New marker for Alzheimer's discovered

Sep 14, 2009

Gothenburg researchers have discovered a previously unknown substance in spinal fluid that can be used to diagnose Alzheimer's disease. The findings, described in a thesis from the Sahlgrenska Academy at the University of ...

Googling brain proteins with 3-D goggles

Feb 15, 2007

The Allen Brain Atlas, a genome-wide map of the mouse brain on the Internet, has been hailed as “Google of the brain.” The atlas now has a companion or the brain’s working molecules, a sort of pop-up ...

Recommended for you

User comments : 0