Overexpression of ARD1A gene reduces tumor size and number in mice

Mar 01, 2010

Overexpression of the ARD1A gene (arrest-defective protein 1225) in mice reduced the number and size of both primary tumors and metastases, researchers report in a new study published online March 1in the Journal of the National Cancer Institute.

ARD1A blocks the expression of VEGFA ( A), an important mediator of blood vessel growth in tumors. It regulates VEGFA expression indirectly by acetylating the transcription factor that induces VEGFA expression— hypoxia inducible factor 1(HIF-1α). Acetylation by ARD1A triggers degradation of HIF-1a, which blocks VEGFA expression and blood vessel proliferation in tumors.

To better understand ARD1A's effect on VEGFA expression and on tumor growth, Goo Taeg Oh, D.V.M., Ph.D., Division of Life and Pharmaceutical Sciences, Ewha Women's University, in Seoul, Korea, and colleagues injected mice that overexpressed mARDIA with human gastric cancer and mouse melanoma cells. They then measured growth, metastasis, and VEGFA expression.

The researchers found that the mARD1A-expressing mice had statistically significantly fewer intestinal polyps than controls. The growth and metastases of transplanted tumors were statistically significantly reduced in mice injected with mARD1A- overexpressing cells compared to injected with control cells. The group also confirmed that overexpression of mARD1A decreases VEGFA expression and microvessel density in tumors and that HIF-1α only needs to be acetylated at one site to be degraded.

The authors write: "…We conclude that mARD1A225 presents a novel target in the regulation of HIF-1a stability and may have potent therapeutic effects in combination with currently approved anti-VEGFA treatments such as bevacizumab."

Explore further: Team finds mutations expressed within melanoma tumors that predict effective responses to a groundbreaking immunotherapy

More information: jnci.oxfordjournals.org

add to favorites email to friend print save as pdf

Related Stories

New hope for cancer comes straight from the heart

Jan 05, 2009

Digitalis-based drugs like digoxin have been used for centuries to treat patients with irregular heart rhythms and heart failure and are still in use today. In the Dec. 16 issue of the Proceedings of the National Academy of ...

Recommended for you

The STING of radiation

2 hours ago

A team of researchers led by Ludwig Chicago's Yang-Xin Fu and Ralph Weichselbaum has uncovered the primary signaling mechanisms and cellular interactions that drive immune responses against tumors treated with radiotherapy. ...

User comments : 0

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.