Dendritic cells as a new player in arteries and heart valves

Feb 17, 2009

(PhysOrg.com) -- In 1973, Ralph M. Steinman launched a new scientific discipline when he published his discovery of the dendritic cell, an odd-shaped player in the immune system. Since then, dendritic cells have proved to be critical sentinels on the lookout for foreign invaders, involved in early immune responses such as graft rejection, resistance to tumors and autoimmune diseases. Now it appears they need to be considered in research on arterial and heart function, too, according to new experiments to be published February 16 in The Journal of Experimental Medicine.

Previous research had turned up indirect evidence of dendritic cells in the large arteries of both mice and people. But Jaehoon Choi, a postdoctoral associate in Steinman’s Laboratory of Cellular Physiology and Immunology at The Rockefeller University, now has proof positive, at least in mice. More importantly, he and colleagues showed that the key immune cells sit just beneath the lining of the aorta and can project their dendrites into the bloodstream to capture foreign materials. “This has been seen in other linings like the airway and the intestine, but never in the major arteries,” Steinman says.

Choi used genetically modified mice developed by Rockefeller Professor Michel C. Nussenzweig’s lab that allowed him to tag a known marker for dendritic cells, a molecule called CD11c, with a fluorescent protein. High levels of CD11c are found in dendritic cells, and the fluorescing protein proved effective for identifying them in the heart. Choi’s experiments showed not only that the tagged dendritic cells had the requisite CD11c, but also turned up evidence of another immune system molecule involved in delivering foreign substances to the T cells that can kill them. Moreover, they isolated dendritic cells from the aorta and found that they were just as effective at handing over foreign substances and stimulating the immune system’s killer cells as those in immune organs such as the spleen.

Still, the question of whether the dendritic cells could do their normal job in the intact heart remained. To test this, Choi injected mice with a model foreign protein, waited 20 hours, and then isolated the dendritic cells. He found that the isolated cells spurred the growth of the appropriate T cells, indicating that they had captured the invaders like they were supposed to and delivered them over for destruction. “We were the first to show the function of the dendritic cell in the aorta and cardiac valves,” Choi says.

Now he is trying to figure out the role of dendritic cells in a mouse model of arteriosclerosis, a hardening of the arteries by plaques filled with lipids such as cholesterol. The recent research found ample evidence of dendritic cells in the aortic root, arch and descending aorta, the same areas that are prone to the disease.

“What does this really mean? Do they handle lipids or stimulate inflammatory response?” Steinman asks. “We don’t know, but that’s what we’d like to find out next.”

More information: The Journal of Experimental Medicine online: February 16, 2009

Provided by Rockefeller University

Explore further: Everything you wanted to know about mitochondrial mutations but were afraid to ask

add to favorites email to friend print save as pdf

Related Stories

Cellulose with Braille for cells

Jan 19, 2015

Artificial implants such as pacemakers often cause complications because the body identifies them as foreign objects. Researchers at ETH Zurich have now demonstrated a simple method to fabricate cellulose-sheaths ...

Extra-short nanowires best for brain

Jan 15, 2015

If in the future electrodes are inserted into the human brain - either for research purposes or to treat diseases - it may be appropriate to give them a 'coat' of nanowires that could make them less irritating for the brain ...

Locking mechanism found for 'scissors' that cut DNA

Dec 24, 2014

Researchers at Johns Hopkins have discovered what keeps an enzyme from becoming overzealous in its clipping of DNA. Since controlled clipping is required for the production of specialized immune system proteins, ...

How did the chicken beat the infection?

Dec 23, 2014

A new Emmy Noether Group in LMU's Faculty of Veterinary Science is dedicated to understanding the mechanisms that underlie the operation of the immune system in chickens.

Recommended for you

New cells may help treat diabetes

10 hours ago

Starting from human skin cells, researchers at the University of Iowa have created human insulin-producing cells that respond to glucose and correct blood-sugar levels in diabetic mice. The findings may represent ...

Using stem cells to grow new hair

Jan 27, 2015

In a new study from Sanford-Burnham Medical Research Institute (Sanford-Burnham), researchers have used human pluripotent stem cells to generate new hair. The study represents the first step toward the development ...

User comments : 0

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.