A further study of Helicobacter pylori reducing gastric blood flow

Jan 21, 2009

A research group from Sweden investigated the mechanisms underlying the reduction in gastric blood flow induced by a luminal water extract of Helicobacter pylori (H. pylori). They found that the H. pylori water extract reduces gastric mucosal blood flow acutely through iNOS- and nerve-mediated pathways.

Gastric mucosal blood flow has a vital role in gastric mucosal protection. A high blood flow is considered a good protection against injury, as it dilutes, neutralizes, and removes hazardous substances that have penetrated the gastric mucosal barrier. A research group in Sweden has previously found that a water extract of H. pylori reduces the mucosal blood flow in rats by a mast cell- and platelet activating factor (PAF)-dependent pathway. In this study they further investigated the mechanisms behind the reduction in blood flow in mice. This will be published on January 14, 2009 in the World Journal of Gastroenterology.

In their study, the stomachs of isoflurane-anesthetized mice were exteriorized, and the mucosal surface exposed. Blood flow was measured with the laser-Doppler technique, and systemic arterial blood pressure monitored. C57BL/6 mice were exposed to water extract produced from H. pylori strain 88-23. To investigate the role of a nerveor iNOS-mediated pathway, they used intraluminal
lidocaine and iNOS-/- mice. Blood flow response to the endogenous nitric oxide synthase inhibitor asymmetric dimethyl arginine (ADMA) was also assessed.

They found that in wild-type mice, the water extract of H. pylori decreased mucosal blood flow by approximately 30%. This reduction was abolished in iNOS-deficient mice, and by pre-treatment with lidocaine. Luminally applied ADMA resulted in reduction in blood flow similar to that observed in wild-type mice exposed to the water extract of H. pylori.

The results indicated that H. pylori water extract reduces gastric mucosal blood flow acutely through an iNOS- and nerve-mediated pathway. This will be very importance to understand the development of gastric inflammation.

Source: World Journal of Gastroenterology

Explore further: Clipping proteins that package genes may limit abnormal cell growth in tumors

add to favorites email to friend print save as pdf

Related Stories

Explainer: What is X-ray crystallography?

Feb 04, 2014

Around 100 years ago a father and his son in north England conducted an experiment that would revolutionise the way scientists study molecules. A refined version of their method still remains one of the most ...

Recommended for you

Organovo has 3D-printed liver tissue for drug testing

Nov 20, 2014

(Medical Xpress)—The commercial release of 3D printed liver tissue was announced earlier this week. Organovo is the company behind the release. The product is intended for use for preclinical drug discovery ...

User comments : 0

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.