Dental pulp cells for stem cell banking

June 17, 2010

Defined sets of factors can reprogram human cells to induced pluripotent stem (iPS) cells. However, many types of human cells are not easily accessible to minimally invasive procedures.

In a paper published in the International and American Associations for Dental Research's , lead researcher K. Tezuka and researchers N. Tamaoki, H. Aoki, T. Takeda-Kawaguchi, K. Iida, T. Kunisada and T. Shibata all from the Gifu University Graduate School of Medicine, Japan; and K. Takahashi, T. Tanaka and S. Yamanaka, all from Kyoto University, Japan, evaluate dental pulp as an optimal source of iPS cells, since they are easily obtained from extracted teeth and can be expanded under simple culture conditions.

From all six cell lines tested with the conventional three or four reprogramming factors, iPS cells were effectively established from five lines. Furthermore, determination of the HLA types of 107 DPC lines revealed two lines homozygous for all three HLA loci and showed that if an iPS bank is established from these initial pools, the bank will cover approximately 20 percent of the Japanese population with a perfect match.

Analysis of these data demonstrates the promising potential of cell collections as a source of cell banks for use in regenerative medicine. Direct reprogramming of patients' would allow for therapy free from immune-mediated rejection. An alternative approach is to establish an iPS cell bank consisting of various human leukocyte antigen (HLA) types. Safety issues must be considered as to which types of somatic cells should be used for such iPS cell banks.

"This work is significant in that it proposes the exciting potential of stem cell banking from readily available extracted teeth," said JDR Editor-in-Chief William Giannobile. "Although at an early stage of development, this innovation offers prospects for cell therapy approaches for the treatment of human disease."

Explore further: Human embryonic stem cell lines created that avoid immune rejection

More information: The complete research study is published in the Journal of Dental Research, and is available online at jdr.sagepub.com/cgi/content/abstract/0022034510366846v1

Related Stories

Researchers generate functional neurons from somatic cells

February 24, 2009

In a new study, researchers were able to generate functionally mature motor neurons from induced pluripotent stem (iPS) cells, which are engineered from adult somatic cells and can differentiate into most other cell types. ...

Broke a tooth? Grow another!

January 13, 2010

(PhysOrg.com) -- To all those who have made deals with the tooth fairy in the past: you probably sold your teeth below their fair value.

Recommended for you

Machine Translates Thoughts into Speech in Real Time

December 21, 2009

(PhysOrg.com) -- By implanting an electrode into the brain of a person with locked-in syndrome, scientists have demonstrated how to wirelessly transmit neural signals to a speech synthesizer. The "thought-to-speech" process ...

Quantum Theory May Explain Wishful Thinking

April 14, 2009

(PhysOrg.com) -- Humans don’t always make the most rational decisions. As studies have shown, even when logic and reasoning point in one direction, sometimes we chose the opposite route, motivated by personal bias or simply ...

0 comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.